Soo Joon Sohn1. 1. Department of Otolaryngology, Catholic University of Daegu School of Medicine, Daegu, South Korea. sjsohn@cu.ac.kr
Abstract
CONCLUSION: SP upregulates osteoclastogenesis by activating NF-kappaB in osteoclast precursors. OBJECTIVE: Osteoclastic bone resorption is a common finding of otitis media with or without cholesteatoma. Factors upregulating osteoclastogenesis are implicated in bone resorption. The aim of this study was to investigate the effect of substance P (SP) on mouse osteoclastogenesis in vitro. MATERIAL AND METHODS: The SP dose response was analyzed in a mouse osteoclast culture induced by macrophage colony-stimulating factor and receptor activator for nuclear factor kappa B (NF-kappaB) ligand. Western blot analysis was performed to elucidate the subcellular target of SP in osteoclastogenesis. RESULTS: Treatment with SP significantly increased osteoclastogenesis compared to control. SP activated nuclear translocation of NF-kappaB, which is an essential event in osteoclastogenesis in osteoclast precursors (bone marrow macrophages).
CONCLUSION: SP upregulates osteoclastogenesis by activating NF-kappaB in osteoclast precursors. OBJECTIVE:Osteoclastic bone resorption is a common finding of otitis media with or without cholesteatoma. Factors upregulating osteoclastogenesis are implicated in bone resorption. The aim of this study was to investigate the effect of substance P (SP) on mouse osteoclastogenesis in vitro. MATERIAL AND METHODS: The SP dose response was analyzed in a mouse osteoclast culture induced by macrophage colony-stimulating factor and receptor activator for nuclear factor kappa B (NF-kappaB) ligand. Western blot analysis was performed to elucidate the subcellular target of SP in osteoclastogenesis. RESULTS: Treatment with SP significantly increased osteoclastogenesis compared to control. SP activated nuclear translocation of NF-kappaB, which is an essential event in osteoclastogenesis in osteoclast precursors (bone marrow macrophages).
Authors: Liping Wang; Xiaoyou Shi; Rong Zhao; Bernard P Halloran; David J Clark; Christopher R Jacobs; Wade S Kingery Journal: Bone Date: 2009-12-02 Impact factor: 4.398
Authors: Liping Wang; Rong Zhao; Xiaoyou Shi; Tzuping Wei; Bernard P Halloran; David J Clark; Christopher R Jacobs; Wade S Kingery Journal: Bone Date: 2009-04-18 Impact factor: 4.398
Authors: Seungyup Sun; Nicklaus H Diggins; Zachary J Gunderson; Jill C Fehrenbacher; Fletcher A White; Melissa A Kacena Journal: Bone Date: 2019-11-09 Impact factor: 4.398