Prostaglandin D2 inhibits IgE-mediated scratching by suppressing histamine release from mast cells.

Effects of prostaglandin (PG) D(2), PGE(2), and PGI(2) on itch-associated scratching responses of mice and histamine release from the rat basophilic leukemia cell line RBL-2H3 were examined. PGD(2) and ketotifen but not PGE(2) and PGI(2) suppressed the scratching caused by ovalbumin injected into ovalbumin-sensitized mice. Ketotifen also suppressed compound 48/80-induced scratching but not PGD(2), PGE(2), and PGI(2). In vitro, PGD(2) suppressed the antigen-induced histamine release from RBL-2H3 cells, but PGE(2) and PGI(2) did not. These findings suggest that PGD(2) specifically suppressed IgE-mediated scratching by inhibiting IgE-mediated histamine release from mast cells.