| Literature DB >> 15878653 |
Patricia Andres1, Alejandro Petroni, Diego Faccone, Fernando Pasterán, Roberto Melano, Melina Rapoport, Mariela Martínez, Catalina Culasso, Adriana Di Bella, Bettina Irigoyen, Jorgelina Mulki, Adriana Procopio, Martha von Specht, Marcelo Galas.
Abstract
A 9-year nation-wide survey of the presence of extended-spectrum beta-lactamases (ESBLs) in Shigella flexneri is described. Ten of 9033 (0.1%) isolates produced ESBLs, which were characterized by isoelectric focusing, PCR and DNA sequencing. These were CTX-M-2 (five isolates), TOHO-1 (one isolate), SHV-2 (two isolates) and PER-2 (two isolates, the first report in S. flexneri world wide). The emergence of each ESBL type in S. flexneri was not restricted to a particular region of Argentina. TOHO-1 showed a more basic isoelectric point (8.4) than that previously found (7.8) and its encoding gene (bla(TOHO-1a)) harboured a silent change, G825A, relative to the reported bla(TOHO-1). All the ESBL-encoding genes were transferred to Escherichia coli by conjugation. PFGE analysis indicated that the 10 ESBL-producing S. flexneri isolates were subtypes of a unique clone.Entities:
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Year: 2005 PMID： 15878653 DOI： 10.1016/j.ijantimicag.2005.02.016
Source DB: PubMed Journal: Int J Antimicrob Agents ISSN： 0924-8579 Impact factor: 5.283