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Literature DB >> 1587551

Activation of Lyt-2 associated with distant upstream insertion of an SL3-3 provirus.

Abstract

Two Lyt-2+ mutants of the T-cell lymphoma SL12.4.10 were selected by fluorescence activated cell sorting. Both mutants expressed Lyt-2 (CD8 alpha-chain) but not Lyt-3 (CD8 beta-chain). Derivatives of one Lyt-2+ mutant that expressed Lyt-3 could be isolated by sorting for Lyt-3+ cells. Southern blotting analysis indicated that both mutants had structural rearrangements within or immediately 3' of the Lyt-3 gene, accompanied by demethylation of at least one Hpa II site within the Lyt-2 gene. Gene cloning analysis of one mutant demonstrated that the structural rearrangement was due to insertion of an SL3-3 provirus 35 kb 5' to the Lyt-2 gene. It is likely that Lyt-2 gene activation is a direct or indirect consequence of proviral insertion at this site.

Entities: Disease Gene

Mesh：

Substances：

Year: 1992 PMID： 1587551 DOI： 10.1007/bf00209286

Source DB: PubMed Journal: Immunogenetics ISSN： 0093-7711 Impact factor: 2.846

55 in total

1. Gene transfer of the Ly-3 chain gene of the mouse CD8 molecular complex: co-transfer with the Ly-2 polypeptide gene results in detectable cell surface expression of the Ly-3 antigenic determinants.

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Authors: O R Choi; J D Engel
Journal: Cell Date: 1988-10-07 Impact factor: 41.582

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7. Segregation of recessive phenotypes in somatic cell hybrids: role of mitotic recombination, gene inactivation, and chromosome nondisjunction.

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8. Expression of transferrin receptor on murine hematopoietic progenitors.

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Journal: Cell Immunol Date: 1984-01 Impact factor: 4.868

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Authors: H A Crissman; J A Steinkamp
Journal: Cytometry Date: 1982-09

10. A Thy-1- mutant defining a gene acting in trans position to regulate cell-surface Thy-1 glycoprotein expression and Thy-1 messenger RNA content.

Authors: R Hyman; K Cunningham
Journal: Immunogenetics Date: 1986 Impact factor: 2.846

1 in total

1. Coordinate change in phenotype in a mouse cell line selected for CD8 expression.

Authors: R Hyman; V Stallings
Journal: Immunogenetics Date: 1992 Impact factor: 2.846