The clathrin interacting protein Clint/epsinR in rat testicular germ cells.

The plasma membrane and the trans-Golgi network (TGN) are major intracellular sites for clathrin-mediated membrane budding. Only recently has the clathrin interacting protein Clint/epsinR/enthoprotin been identified, which is thought to be involved in clathrin-dependent membrane budding from the TGN. Using immunocytochemistry, we now report the presence of Clint in the Golgi region of spermatocytes and spermatids of the rat testis. Together with subcellular fractionation experiments, our data show that, in male germ cells, Clint behaves as a peripheral membrane protein that is probably involved in TGN-related vesicle budding. Moreover, the immunostaining of the acrosome in round and elongating spermatids indicates that Clint operates in membrane traffic between the TGN and the acrosome. It may thus be speculated that the protein is involved in the biogenesis and shaping of acrosomal membranes.