BACKGROUND: The external auditory canal cholesteatoma (EACC) is characterized by hyperproliferation of the epithelial and subepithelial tissue. Compared to normal meatal skin, strong expression of FGF-2 and VEGF had previously been detected. Many authors reported that FGF-2 acts via VEGF and is induced by hypoxia. Hypoxia seems to be pivotal for establishing EACC. Therefore, human EACC fibroblasts were investigated by incubating with FGF-2 and determining VEGF. PATIENTS AND METHODS: We harvested fibroblasts from human EACC and normal meatal skin and incubated the fibroblast culture with 50 ng/ml FGF-2 and determined VEGF concentrations after 1-4 days. RESULTS: Compared to untreated fibroblast cultures, there was a significant increase of VEGF concentration (p<0.05). However, there was no significant difference between the proliferation quantities. CONCLUSION: VEGF and FGF-2 are possibly involved in a cascade of growth factor activities, which modulates their concentration in human-derived EACC fibroblast culture. Exogenous FGF-2 increased fibroblast expression for VEGF, which is a major autocrine mediator of FGF-2-induced angiogenesis and proliferation.
BACKGROUND: The external auditory canal cholesteatoma (EACC) is characterized by hyperproliferation of the epithelial and subepithelial tissue. Compared to normal meatal skin, strong expression of FGF-2 and VEGF had previously been detected. Many authors reported that FGF-2 acts via VEGF and is induced by hypoxia. Hypoxia seems to be pivotal for establishing EACC. Therefore, human EACC fibroblasts were investigated by incubating with FGF-2 and determining VEGF. PATIENTS AND METHODS: We harvested fibroblasts from human EACC and normal meatal skin and incubated the fibroblast culture with 50 ng/ml FGF-2 and determined VEGF concentrations after 1-4 days. RESULTS: Compared to untreated fibroblast cultures, there was a significant increase of VEGF concentration (p<0.05). However, there was no significant difference between the proliferation quantities. CONCLUSION:VEGF and FGF-2 are possibly involved in a cascade of growth factor activities, which modulates their concentration in human-derived EACC fibroblast culture. Exogenous FGF-2 increased fibroblast expression for VEGF, which is a major autocrine mediator of FGF-2-induced angiogenesis and proliferation.
Authors: J DiSalvo; M L Bayne; G Conn; P W Kwok; P G Trivedi; D D Soderman; T M Palisi; K A Sullivan; K A Thomas Journal: J Biol Chem Date: 1995-03-31 Impact factor: 5.157
Authors: G Seghezzi; S Patel; C J Ren; A Gualandris; G Pintucci; E S Robbins; R L Shapiro; A C Galloway; D B Rifkin; P Mignatti Journal: J Cell Biol Date: 1998-06-29 Impact factor: 10.539