Literature DB >> 15867962

Multiplex-PCR for detection of natural Leishmania infection in Lutzomyia spp. captured in an endemic region for cutaneous leishmaniasis in state of Sucre, Venezuela.

Alicia Jorquera1, Ricardo González, Edgar Marchán-Marcano, Milagros Oviedo, Mercedes Matos.   

Abstract

We studied the natural infection of Lutzomyia (Lutzomyia) sp. with Leishmania in endemic foci of cutaneous leishmaniasis in the Paria peninsula, state of Sucre, Venezuela. Sand flies were collected between March 2001 and June 2003, using Shannon light-traps and human bait. Of the 1291 insects captured, only two species of phlebotomines were identified: L. ovallesi (82.75%) and L. gomezi (17.42%). A sample of the collected sand flies (51 pools of 2-12 individuals) were analyzed by using a multiplex-PCR assay for simultaneous detection of New Word Leishmaniaand Viannia subgenera. The results showed a total of 8 pools (15.68%) infected; of these, 7 were L. ovallesi naturally infected with L. braziliensis (2 pools) and L. mexicana (5 pools) and 1 pool of L. gomezi infected by L. braziliensis.

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Year:  2005        PMID： 15867962     DOI： 10.1590/s0074-02762005000100008

Source DB:  PubMed          Journal:  Mem Inst Oswaldo Cruz        ISSN： 0074-0276            Impact factor:   2.743


  16 in total

Review 1.  Molecular Diagnosis of Visceral Leishmaniasis.

Authors:  Shyam Sundar; Om Prakash Singh
Journal:  Mol Diagn Ther       Date:  2018-08       Impact factor: 4.074

2.  Leishmania spp. Infection Rate and Feeding Patterns of Sand Flies (Diptera: Psychodidae) from a Hyperendemic Cutaneous Leishmaniasis Community in Panamá.

Authors:  Chystrie A Rigg; José E Calzada; Azael Saldaña; Milixa Perea; Luis F Chaves; Anayansi Valderrama
Journal:  Am J Trop Med Hyg       Date:  2019-04       Impact factor: 2.345

Review 3.  Molecular epidemiology for vector research on leishmaniasis.

Authors:  Hirotomo Kato; Eduardo A Gomez; Abraham G Cáceres; Hiroshi Uezato; Tatsuyuki Mimori; Yoshihisa Hashiguchi
Journal:  Int J Environ Res Public Health       Date:  2010-03-05       Impact factor: 3.390

4.  Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus.

Authors:  Anayansi Valderrama; Mara Garcia Tavares; Jose Dilermando Andrade Filho
Journal:  Parasit Vectors       Date:  2014-01-08       Impact factor: 3.876

Review 5.  Nanodiagnostics in leishmaniasis: A new frontiers for early elimination.

Authors:  Mallikarjuna Rao Gedda; Prasoon Madhukar; Ashish Shukla; Shyam Lal Mudavath; Onkar Nath Srivastava; Om Prakash Singh; Shyam Sundar
Journal:  Wiley Interdiscip Rev Nanomed Nanobiotechnol       Date:  2020-11-03

6.  A real-time PCR assay to estimate Leishmania chagasi load in its natural sand fly vector Lutzomyia longipalpis.

Authors:  Shalindra Ranasinghe; Matthew E Rogers; James G C Hamilton; Paul A Bates; Rhayza D C Maingon
Journal:  Trans R Soc Trop Med Hyg       Date:  2008-05-23       Impact factor: 2.184

7.  Canine leishmaniosis in South America.

Authors:  Filipe Dantas-Torres
Journal:  Parasit Vectors       Date:  2009-03-26       Impact factor: 3.876

8.  Effect of environmental disturbance on the population of sandflies and leishmania transmission in an endemic area of Venezuela.

Authors:  Elsa Nieves; Luzmary Oraá; Yorfer Rondón; Mireya Sánchez; Yetsenia Sánchez; Masyelly Rojas; Maritza Rondón; Maria Rujano; Nestor González; Dalmiro Cazorla
Journal:  J Trop Med       Date:  2014-04-07

9.  Species composition and seasonal abundance of sandflies (Diptera: Psychodidae: Phlebotominae) in coffee agroecosystems.

Authors:  Jeanneth Pérez; Armando Virgen; Julio Cesar Rojas; Eduardo Alfonso Rebollar-Téllez; Castillo Alfredo; Francisco Infante; Oscar Mikery; Carlos Felix Marina; Sergio Ibáñez-Bernal
Journal:  Mem Inst Oswaldo Cruz       Date:  2013-11-04       Impact factor: 2.743

10.  Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis.

Authors:  Suênia da Cunha Gonçalves-de-Albuquerque; Rômulo Pessoa E Silva; Rayana Carla Silva de Morais; Lays Adrianne Mendonça Trajano-Silva; Carlos Gustavo Régis-da-Silva; Sinval Pinto Brandão-Filho; Milena de Paiva-Cavalcanti
Journal:  J Venom Anim Toxins Incl Trop Dis       Date:  2014-04-22
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