Literature DB >> 15866952

Localization of chaperones DnaK and GroEL in bacterial inclusion bodies.

M Mar Carrió1, Antonio Villaverde.   

Abstract

By immunostaining and transmission electron microscopy, chaperones DnaK and GroEL have been identified at the solvent-exposed surface of bacterial inclusion bodies and entrapped within these aggregates, respectively. Functional implications of this distinct localization are discussed in the context of Escherichia coli protein quality control.

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Year:  2005        PMID: 15866952      PMCID: PMC1111989          DOI: 10.1128/JB.187.10.3599-3601.2005

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  18 in total

1.  Isolating inclusion bodies from bacteria.

Authors:  G Georgiou; P Valax
Journal:  Methods Enzymol       Date:  1999       Impact factor: 1.600

2.  Protein aggregation as bacterial inclusion bodies is reversible.

Authors:  M M Carrió; A Villaverde
Journal:  FEBS Lett       Date:  2001-01-26       Impact factor: 4.124

3.  Monitoring of genes that respond to overproduction of an insoluble recombinant protein in Escherichia coli glucose-limited fed-batch fermentations.

Authors:  B Jürgen; H Y Lin; S Riemschneider; C Scharf; P Neubauer; R Schmid; M Hecker; T Schweder
Journal:  Biotechnol Bioeng       Date:  2000-10-20       Impact factor: 4.530

4.  Role of molecular chaperones in inclusion body formation.

Authors:  M Mar Carrió; Antonio Villaverde
Journal:  FEBS Lett       Date:  2003-02-27       Impact factor: 4.124

5.  Protein compositional analysis of inclusion bodies produced in recombinant Escherichia coli.

Authors:  U Rinas; J E Bailey
Journal:  Appl Microbiol Biotechnol       Date:  1992-08       Impact factor: 4.813

Review 6.  Construction and deconstruction of bacterial inclusion bodies.

Authors:  M M Carrió; A Villaverde
Journal:  J Biotechnol       Date:  2002-06-13       Impact factor: 3.307

7.  Dynamics of in vivo protein aggregation: building inclusion bodies in recombinant bacteria.

Authors:  M M Carrió; J L Corchero; A Villaverde
Journal:  FEMS Microbiol Lett       Date:  1998-12-01       Impact factor: 2.742

8.  Influence of the GroE molecular chaperone machine on the in vitro refolding of Escherichia coli beta-galactosidase.

Authors:  A Ayling; F Baneyx
Journal:  Protein Sci       Date:  1996-03       Impact factor: 6.725

9.  Molecular characterization of beta-lactamase inclusion bodies produced in Escherichia coli. 1. Composition.

Authors:  P Valax; G Georgiou
Journal:  Biotechnol Prog       Date:  1993 Sep-Oct

10.  Unscrambling an egg: protein disaggregation by AAA+ proteins.

Authors:  Jimena Weibezahn; Bernd Bukau; Axel Mogk
Journal:  Microb Cell Fact       Date:  2004-01-16       Impact factor: 5.328

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  38 in total

1.  Two distinct states of Escherichia coli cells that overexpress recombinant heterogeneous β-galactosidase.

Authors:  Yun Zhao; Wei He; Wei-Feng Liu; Chun-Chun Liu; Li-Kui Feng; Lei Sun; Yong-Bin Yan; Hai-Ying Hang
Journal:  J Biol Chem       Date:  2012-02-02       Impact factor: 5.157

2.  Localization of functional polypeptides in bacterial inclusion bodies.

Authors:  Elena García-Fruitós; Anna Arís; Antonio Villaverde
Journal:  Appl Environ Microbiol       Date:  2006-11-03       Impact factor: 4.792

3.  Effects of SecE depletion on the inner and outer membrane proteomes of Escherichia coli.

Authors:  Louise Baars; Samuel Wagner; David Wickström; Mirjam Klepsch; A Jimmy Ytterberg; Klaas J van Wijk; Jan-Willem de Gier
Journal:  J Bacteriol       Date:  2008-02-22       Impact factor: 3.490

4.  Rehosting of bacterial chaperones for high-quality protein production.

Authors:  Mónica Martínez-Alonso; Verónica Toledo-Rubio; Rob Noad; Ugutz Unzueta; Neus Ferrer-Miralles; Polly Roy; Antonio Villaverde
Journal:  Appl Environ Microbiol       Date:  2009-10-09       Impact factor: 4.792

5.  Cross-system excision of chaperone-mediated proteolysis in chaperone-assisted recombinant protein production.

Authors:  Mónica Martínez-Alonso; Antonio Villaverde; Neus Ferrer-Miralles
Journal:  Bioeng Bugs       Date:  2009-12-29

Review 6.  Protein folding and aggregation in bacteria.

Authors:  Raimon Sabate; Natalia S de Groot; Salvador Ventura
Journal:  Cell Mol Life Sci       Date:  2010-04-01       Impact factor: 9.261

7.  Two Outer Membrane Proteins Contribute to Caulobacter crescentus Cellular Fitness by Preventing Intracellular S-Layer Protein Accumulation.

Authors:  K Wesley Overton; Dan M Park; Mimi C Yung; Alice C Dohnalkova; John Smit; Yongqin Jiao
Journal:  Appl Environ Microbiol       Date:  2016-09-23       Impact factor: 4.792

8.  Quality control of inclusion bodies in Escherichia coli.

Authors:  Britta Jürgen; Antje Breitenstein; Vlada Urlacher; Knut Büttner; Hongying Lin; Michael Hecker; Thomas Schweder; Peter Neubauer
Journal:  Microb Cell Fact       Date:  2010-05-28       Impact factor: 5.328

Review 9.  Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters.

Authors:  Norma A Valdez-Cruz; Luis Caspeta; Néstor O Pérez; Octavio T Ramírez; Mauricio A Trujillo-Roldán
Journal:  Microb Cell Fact       Date:  2010-03-19       Impact factor: 5.328

Review 10.  Side effects of chaperone gene co-expression in recombinant protein production.

Authors:  Mónica Martínez-Alonso; Elena García-Fruitós; Neus Ferrer-Miralles; Ursula Rinas; Antonio Villaverde
Journal:  Microb Cell Fact       Date:  2010-09-02       Impact factor: 5.328

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