BACKGROUND: In order to study the genotype variation of Sporothrix schenckii, we previously analyzed the genomic sequences of the DNA topoisomerase II genes of this fungus and S. schenckii-related species, such as S. schenckii var. luriei and Ceratocystis stenoceras. OBJECTIVE: To develop a PCR-based identification system that can distinguish S. schenckii from its related species for clinical diagnosis of sporotrichosis. METHODS: Based on the nucleotide sequences of the DNA topoisomerase II genes of S. schenckii, S. schenckii var. luriei and C. stenoceras, three gene-specific primers (SSHF31 and SSHR97 for S. schenckii, and SSLF64 for S. schenckii var. luriei) were designed and evaluated for their specificities in PCR amplifications. RESULTS: The primer set SSHF31/SSHR97 amplified PCR products of 663-817 bp from S. schenckii and approximately 660 bp from S. schenckii var. luriei, while SSLF64/SSHR97 exclusively amplified a major product of 305 bp from S. schenckii var. luriei. CONCLUSION: PCR targeting the DNA topoisomerase II gene specifically and rapidly distinguished S. schenckii from its related species.
BACKGROUND: In order to study the genotype variation of Sporothrix schenckii, we previously analyzed the genomic sequences of the DNA topoisomerase II genes of this fungus and S. schenckii-related species, such as S. schenckii var. luriei and Ceratocystis stenoceras. OBJECTIVE: To develop a PCR-based identification system that can distinguish S. schenckii from its related species for clinical diagnosis of sporotrichosis. METHODS: Based on the nucleotide sequences of the DNA topoisomerase II genes of S. schenckii, S. schenckii var. luriei and C. stenoceras, three gene-specific primers (SSHF31 and SSHR97 for S. schenckii, and SSLF64 for S. schenckii var. luriei) were designed and evaluated for their specificities in PCR amplifications. RESULTS: The primer set SSHF31/SSHR97 amplified PCR products of 663-817 bp from S. schenckii and approximately 660 bp from S. schenckii var. luriei, while SSLF64/SSHR97 exclusively amplified a major product of 305 bp from S. schenckii var. luriei. CONCLUSION: PCR targeting the DNA topoisomerase II gene specifically and rapidly distinguished S. schenckii from its related species.
Authors: L H M de Miranda; L P Quintella; I B dos Santos; R C Menezes; F B Figueiredo; I D F Gremião; T Okamoto; R V C de Oliveira; S A Pereira; R Tortelly; T M P Schubach Journal: Mycopathologia Date: 2009-04-10 Impact factor: 2.574
Authors: Regina Casz Schechtman; Eduardo Mastrangelo Marinho Falcão; Marciela Carard; Maria Salomé Cajas García; Diana Stohmann Mercado; Roderick James Hay Journal: An Bras Dermatol Date: 2021-12-08 Impact factor: 1.896
Authors: Alexandre A Sasaki; Geisa F Fernandes; Anderson M Rodrigues; Fábio M Lima; Marjorie M Marini; Luciano Dos S Feitosa; Marcus de Melo Teixeira; Maria Sueli Soares Felipe; José Franco da Silveira; Zoilo P de Camargo Journal: PLoS One Date: 2014-01-23 Impact factor: 3.240