Literature DB >> 15862463

The use of Chinese hamster ovary (CHO) cells in the study of ion channels.

Nikita Gamper1, James D Stockand, Mark S Shapiro.   

Abstract

The line of epithelial-like Chinese hamster ovary (CHO) cells was initiated by T.T. Puck in 1957. Since then, CHO cells have become a widely used mammalian expression system in industry and science. This paper discusses the different features of CHO cell physiology as well as the specific aspects of using these cells for ion channel studies; among the discussed features are the culturing and transfection of CHO cells, details of electrophysiological recordings from them and applications for the study of ion channel physiology and pharmacology. Examples of successful reconstitution of mammalian ion channels in CHO cells discussed in the paper include reconstitution of KCNQ channel regulation by muscarinic acetylcholine receptors and the study of the amiloride-sensitivity of epithelial sodium channels (ENaC).

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Year:  2005        PMID： 15862463     DOI： 10.1016/j.vascn.2004.08.008

Source DB:  PubMed          Journal:  J Pharmacol Toxicol Methods        ISSN： 1056-8719            Impact factor:   1.950


  42 in total

1.  Determinants within the turret and pore-loop domains of KCNQ3 K+ channels governing functional activity.

Authors:  Oleg Zaika; Ciria C Hernandez; Manjot Bal; Gleb P Tolstykh; Mark S Shapiro
Journal:  Biophys J       Date:  2008-09-12       Impact factor: 4.033

2.  Cannabidiol protects against high glucose-induced oxidative stress and cytotoxicity in cardiac voltage-gated sodium channels.

Authors:  Mohamed A Fouda; Mohammad-Reza Ghovanloo; Peter C Ruben
Journal:  Br J Pharmacol       Date:  2020-03-10       Impact factor: 8.739

3.  Ryanodine and IP3 receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation.

Authors:  Gleb P Tolstykh; Cory A Olsovsky; Bennett L Ibey; Hope T Beier
Journal:  Neurophotonics       Date:  2017-04-05       Impact factor: 3.593

4.  Analysis of plasma membrane integrity by fluorescent detection of Tl(+) uptake.

Authors:  Angela M Bowman; Olena M Nesin; Olga N Pakhomova; Andrei G Pakhomov
Journal:  J Membr Biol       Date:  2010-07-11       Impact factor: 1.843

5.  AKAP79/150 signal complexes in G-protein modulation of neuronal ion channels.

Authors:  Jie Zhang; Manjot Bal; Sonya Bierbower; Oleg Zaika; Mark S Shapiro
Journal:  J Neurosci       Date:  2011-05-11       Impact factor: 6.167

6.  Angiotensin II regulates neuronal excitability via phosphatidylinositol 4,5-bisphosphate-dependent modulation of Kv7 (M-type) K+ channels.

Authors:  Oleg Zaika; Lucienne S Lara; Nikita Gamper; Donald W Hilgemann; David B Jaffe; Mark S Shapiro
Journal:  J Physiol       Date:  2006-06-15       Impact factor: 5.182

7.  Ca2+/calmodulin disrupts AKAP79/150 interactions with KCNQ (M-Type) K+ channels.

Authors:  Manjot Bal; Jie Zhang; Ciria C Hernandez; Oleg Zaika; Mark S Shapiro
Journal:  J Neurosci       Date:  2010-02-10       Impact factor: 6.167

8.  Primary pathways of intracellular Ca(2+) mobilization by nanosecond pulsed electric field.

Authors:  Iurii Semenov; Shu Xiao; Andrei G Pakhomov
Journal:  Biochim Biophys Acta       Date:  2012-12-05

9.  Homomeric and heteromeric assembly of KCNQ (Kv7) K+ channels assayed by total internal reflection fluorescence/fluorescence resonance energy transfer and patch clamp analysis.

Authors:  Manjot Bal; Jie Zhang; Oleg Zaika; Ciria C Hernandez; Mark S Shapiro
Journal:  J Biol Chem       Date:  2008-09-11       Impact factor: 5.157

10.  Affinity for phosphatidylinositol 4,5-bisphosphate determines muscarinic agonist sensitivity of Kv7 K+ channels.

Authors:  Ciria C Hernandez; Björn Falkenburger; Mark S Shapiro
Journal:  J Gen Physiol       Date:  2009-11       Impact factor: 4.086
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