OBJECTIVE: In order to study estrogen-like effect of compounds of Mercury, Chromium and Manganese. METHODS: Mercury Chloride, Manganese Sulfate, Chromium Chloride and Chromium trioxide were selected to perform proliferation assay of MCF-7 human breast cancer cells and binding assay of estrogen receptor for rat uterine. RESULTS: Mercury Chloride could stimulate the proliferation of MCF-7 cell, the maximal increase of MCF-7 cells was measured by Mercury Chloride at 10(-7) mol/L. The effect was blocked completely by a pure antigestrogen ICI182, 780; but Mercury Chloride could not bind to ER by competing with E2. Chromium Chloride, Chromium Trioxide and Manganese Sulfate were not able to stimulate the proliferation of MCF-7 cell and bind to ER through competing against 3H-E2 in vitro. CONCLUSION: Mercury Chloride exhibited the estrogenicity through binding and activating ER; Chromium Chloride, Chromium trioxide and Manganese Sulfate did not show any estrogenicity.
OBJECTIVE: In order to study estrogen-like effect of compounds of Mercury, Chromium and Manganese. METHODS:Mercury Chloride, Manganese Sulfate, Chromium Chloride and Chromium trioxide were selected to perform proliferation assay of MCF-7 humanbreast cancer cells and binding assay of estrogen receptor for rat uterine. RESULTS:Mercury Chloride could stimulate the proliferation of MCF-7 cell, the maximal increase of MCF-7 cells was measured by Mercury Chloride at 10(-7) mol/L. The effect was blocked completely by a pure antigestrogen ICI182, 780; but Mercury Chloride could not bind to ER by competing with E2. Chromium Chloride, Chromium Trioxide and Manganese Sulfate were not able to stimulate the proliferation of MCF-7 cell and bind to ER through competing against 3H-E2 in vitro. CONCLUSION:Mercury Chloride exhibited the estrogenicity through binding and activating ER; Chromium Chloride, Chromium trioxide and Manganese Sulfate did not show any estrogenicity.