PURPOSE: To demonstrate the feasibility of an inverse mushroom-shaped nonmechanical corneal trephination using a femtosecond laser in a noncontact manner. DESIGN: Experimental study. METHODS: In this laboratory study, 10 polymethylmethacrylate (PMMA) blocks and 20 porcine corneas were treated with an industrial femtosecond laser source. The trephination profile consisted of (1) a 7- or 6-mm diameter cylinder from the anterior chamber, (2) an intermediate horizontal connecting plane, and (3) a concentric 5- or 4-mm diameter cylinder upwards. RESULTS: Applying appropriate combinations of pulse energy and spacing, trephination took less than 60 seconds. In porcine eyes, light microscopy displayed trephination edges delineated by partly confluent gas bubbles (10-40 mum) with tissue bridges in between. By TEM, the cut edges were lined by a delicate, electron-dense layer (5-40 nm). CONCLUSIONS: Femtosecond laser technology seems to offer a promising approach towards minimally invasive self-sealing "no-stitch keratoplasty."
PURPOSE: To demonstrate the feasibility of an inverse mushroom-shaped nonmechanical corneal trephination using a femtosecond laser in a noncontact manner. DESIGN: Experimental study. METHODS: In this laboratory study, 10 polymethylmethacrylate (PMMA) blocks and 20 porcine corneas were treated with an industrial femtosecond laser source. The trephination profile consisted of (1) a 7- or 6-mm diameter cylinder from the anterior chamber, (2) an intermediate horizontal connecting plane, and (3) a concentric 5- or 4-mm diameter cylinder upwards. RESULTS: Applying appropriate combinations of pulse energy and spacing, trephination took less than 60 seconds. In porcine eyes, light microscopy displayed trephination edges delineated by partly confluent gas bubbles (10-40 mum) with tissue bridges in between. By TEM, the cut edges were lined by a delicate, electron-dense layer (5-40 nm). CONCLUSIONS: Femtosecond laser technology seems to offer a promising approach towards minimally invasive self-sealing "no-stitch keratoplasty."
Authors: G Gerten; T Ripken; P Breitenfeld; R R Krueger; O Kermani; H Lubatschowski; U Oberheide Journal: Ophthalmologe Date: 2007-01 Impact factor: 1.059
Authors: S J Linke; L Ren; A Frings; J Steinberg; W Wöllmer; T Katz; R Reimer; N O Hansen; N Jowett; G Richard; R J Dwayne Miller Journal: Ophthalmologe Date: 2014-06 Impact factor: 1.059