Literature DB >> 15858294

Detection of rabies-specific antigens by egg yolk antibody (IgY) to the recombinant rabies virus proteins produced in Escherichia coli.

Yurie Motoi1, Satoshi Inoue, Hajime Hatta, Kozue Sato, Kinjiro Morimoto, Akio Yamada.   

Abstract

We obtained rabies-specific egg yolk antibodies (IgY) by immunizing hens with recombinant His-tagged nucleoprotein and phosphoprotein (rN, rP) of the rabies virus (CVS-11 strain) expressed in Escherichia coli. The anti-rN and rP IgY were shown to bind specifically to the respective proteins of the CVS-11 strain of rabies virus by Western blotting, immune fluorescent assay and immunohistochemistry, indicating that IgY to rabies recombinant proteins could serve as a reagent for diagnosis of rabies virus infection.

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Year:  2005        PMID： 15858294

Source DB:  PubMed          Journal:  Jpn J Infect Dis        ISSN： 1344-6304            Impact factor:   1.362


  3 in total

1.  Development of hen antihepatitis B antigen IgY-based conjugate for ELISA assay.

Authors:  Najat Muayed Nafea; Majeed Arsheed Sabbah; Raghad Al-Suhail; Amir Hossein Mahdavi; Sedigheh Asgary
Journal:  Adv Biomed Res       Date:  2015-05-11

2.  Development and evaluation of an anti-rabies virus phosphoprotein-specific monoclonal antibody for detection of rabies neutralizing antibodies using RFFIT.

Authors:  Jihye Um; Byung Chul Chun; Yeong Seon Lee; Kyu Jam Hwang; Dong-Kun Yang; Jun-Sun Park; Su Yeon Kim
Journal:  PLoS Negl Trop Dis       Date:  2017-12-21

3.  Preparation of Anti-Rabies Virus N Protein IgYs by DNA Immunization of Hens Using Different Types of Adjuvants.

Authors:  Nanase Kubo; Mari Nishii; Satoshi Inoue; Akira Noguchi; Hajime Hatta
Journal:  J Poult Sci       Date:  2022-04-25       Impact factor: 1.768
  3 in total

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