Literature DB >> 15858138

Molecular typing of West Nile Virus, Dengue, and St. Louis encephalitis using multiplex sequencing.

Thuraiayah Vinayagamoorthy1, Kirk Mulatz, Michael Drebot, Roger Hodkinson.   

Abstract

We report the development of an assay to simultaneously identify three of the clinically important flaviviruses (West Nile Virus, Dengue, and St. Louis encephalitis). This assay is based on the nucleotide sequence variations within a 266-bp region of the non-structural protein 5. Further, based on the nucleotide variations in the same region of the non-structural protein 5, four of the present Dengue serotypes were identified. To identify some of the subtypes of WNV we have developed a second assay using multiplex sequencing technology. The format of the result of this assay is an electropherogram of two genomic segments of the WNV genome: a 48-nucleotide sequence from the anchored core protein C and a 45-nucleotide sequence coding for the non-structural proteins (proteinase and putative helicase genes).

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Year:  2005        PMID： 15858138      PMCID： PMC1867533          DOI： 10.1016/S1525-1578(10)60541-7

Source DB:  PubMed          Journal:  J Mol Diagn        ISSN： 1525-1578            Impact factor:   5.568


  27 in total

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Authors:  R A Hall; J H Scherret; J S Mackenzie
Journal:  Ann N Y Acad Sci       Date:  2001-12       Impact factor: 5.691

2.  Detection of West Nile virus antigen in mosquitoes and avian tissues by a monoclonal antibody-based capture enzyme immunoassay.

Authors:  Ann R Hunt; Roy A Hall; Amy J Kerst; Roger S Nasci; Harry M Savage; Nicholas A Panella; Kristy L Gottfried; Kristen L Burkhalter; John T Roehrig
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

3.  West Nile virus and blood donors.

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4.  [Analysis of new variants of West Nile fever virus].

Authors:  A G Prilipov; R M Kinney; E I Samokhvalov; H M Savage; S V Al'khovskiĭ; K R Tsuchiya; V L Gromashevskiĭ; G K Sadykova; A G Shatalov; O I Vyshemirskiĭ; E V Usachev; V V Mokhonov; A G Voronina; A M Butenko; V F Larichev; A N Zhukov; A I Kovtunov; D J Gubler; D K L'vov
Journal:  Vopr Virusol       Date:  2002 Jul-Aug

5.  West Nile encephalitis epidemic in southeastern Romania.

Authors:  T F Tsai; F Popovici; C Cernescu; G L Campbell; N I Nedelcu
Journal:  Lancet       Date:  1998-09-05       Impact factor: 79.321

6.  Diagnostic methods applied to analysis of an outbreak of equine influenza in a riding school in which vaccine failure occurred.

Authors:  C van Maanen; G J van Essen; J Minke; J M Daly; P J Yates
Journal:  Vet Microbiol       Date:  2003-06-10       Impact factor: 3.293

7.  The relationships between West Nile and Kunjin viruses.

Authors:  J H Scherret; M Poidinger; J S Mackenzie; A K Broom; V Deubel; W I Lipkin; T Briese; E A Gould; R A Hall
Journal:  Emerg Infect Dis       Date:  2001 Jul-Aug       Impact factor: 6.883

8.  A replication-efficient mutant of West Nile virus is insensitive to DI particle interference.

Authors:  M A Brinton; A V Fernandez
Journal:  Virology       Date:  1983-08       Impact factor: 3.616

9.  Prevalence of West Nile virus infection in India.

Authors:  J P Thakare; T L G Rao; V S Padbidri
Journal:  Southeast Asian J Trop Med Public Health       Date:  2002-12       Impact factor: 0.267

10.  Experimental infection of North American birds with the New York 1999 strain of West Nile virus.

Authors:  Nicholas Komar; Stanley Langevin; Steven Hinten; Nicole Nemeth; Eric Edwards; Danielle Hettler; Brent Davis; Richard Bowen; Michel Bunning
Journal:  Emerg Infect Dis       Date:  2003-03       Impact factor: 6.883
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