D Aubin1, A Gagnon, A Sorisky. 1. Department of Medicine and of Biochemistry, Microbiology and Immunology, Ottawa Health Research Institute, Ottawa Hospital, University of Ottawa, Ottawa, ON, Canada.
Abstract
OBJECTIVE: Phosphoinositide 3-kinase (PI3K) is required for murine adipocyte differentiation. However, a recent report concluded that PI3K was not involved in the differentiation of human preadipocytes into adipocytes. We have re-examined the role of PI3K in human preadipocyte differentiation, enrolling more patients and using more adipogenic indices. METHODS: Human preadipocytes, isolated from nine patients, were induced to differentiate in the presence or absence of 100 nM wortmannin. After 12-15 days, triacylglycerol accumulation and the expression of adipogenic markers (fatty acid synthase and adiponectin) were measured. RESULTS: A significant inhibition in triacylglycerol accumulation and in the induction of fatty acid synthase protein expression was observed, but there was no effect on adiponectin protein expression. CONCLUSION: Inhibition of PI3K reduces the differentiation of human preadipocytes into adipocytes, suggesting a role for this enzyme in the human adipogenic process.
OBJECTIVE:Phosphoinositide 3-kinase (PI3K) is required for murine adipocyte differentiation. However, a recent report concluded that PI3K was not involved in the differentiation of human preadipocytes into adipocytes. We have re-examined the role of PI3K in human preadipocyte differentiation, enrolling more patients and using more adipogenic indices. METHODS:Human preadipocytes, isolated from nine patients, were induced to differentiate in the presence or absence of 100 nM wortmannin. After 12-15 days, triacylglycerol accumulation and the expression of adipogenic markers (fatty acid synthase and adiponectin) were measured. RESULTS: A significant inhibition in triacylglycerol accumulation and in the induction of fatty acid synthase protein expression was observed, but there was no effect on adiponectin protein expression. CONCLUSION: Inhibition of PI3K reduces the differentiation of human preadipocytes into adipocytes, suggesting a role for this enzyme in the human adipogenic process.
Authors: Kelly Cleveland-Donovan; Laura A Maile; William G Tsiaras; Tamara Tchkonia; James L Kirkland; Charlotte M Boney Journal: Endocrinology Date: 2010-06-16 Impact factor: 4.736
Authors: Kishan Kumar Chudasama; Jonathon Winnay; Stefan Johansson; Tor Claudi; Rainer König; Ingfrid Haldorsen; Bente Johansson; Ju Rang Woo; Dagfinn Aarskog; Jørn V Sagen; C Ronald Kahn; Anders Molven; Pål Rasmus Njølstad Journal: Am J Hum Genet Date: 2013-06-27 Impact factor: 11.025
Authors: Xiao Luo; Louise J Hutley; Julie A Webster; Yu-Hee Kim; Dong-Fang Liu; Felicity S Newell; Charlotte H Widberg; Anthony Bachmann; Nigel Turner; Carsten Schmitz-Peiffer; Johannes B Prins; Gong-She Yang; Jonathan P Whitehead Journal: Diabetes Date: 2012-01 Impact factor: 9.461
Authors: Miriane de Oliveira; Renata de Azevedo Melo Luvizotto; Regiane Marques Castro Olimpio; Maria Teresa De Sibio; Sandro José Conde; Carolina Biz Rodrigues Silva; Fernanda Cristina Fontes Moretto; Célia Regina Nogueira Journal: PLoS One Date: 2013-09-18 Impact factor: 3.240