Teresa Adell1, Werner E G Müller. 1. Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Duesbergweg 6, D-55099 Mainz, Germany.
Abstract
BACKGROUND INFORMATION: T-box transcription factors are a large family of transcriptional regulators involved in many aspects of embryonic development. In a previous report, we described the isolation and genomic characterization of two T-box genes from the siliceous sponge Suberites domuncula: a Brachyury homologue, Sd-Bra, and a Tbx2 homologue, Sd-Tbx2. Elucidation of the genomic structure of Sd-Bra allowed us to demonstrate the existence of two different isoforms, resulting from alternative splicing. Moreover, we demonstrated that the shorter isoform exists in two different glycosylation states. RESULTS: In the present study, we demonstrate a differential subcellular localization of the three Sd-Bra isoforms, suggesting that its differential nuclear import could be an important mechanism for its functional regulation. Furthermore, we demonstrate that Sd-Tbx2 exists only in one isoform, which is mainly localized in the nucleus. The pattern of expression of Sd-Bra and Sd-Tbx2 genes is analysed in sponge tissue, in gemmules and in cultured cells. CONCLUSION: These results suggest a conserved role for Sd-Bra in the control of morphogenetic movements through the regulation of cell-adhesion properties and the involvement of Sd-Tbx2 in the determination of cell identity in the early stages of differentiation, reminiscent of the function of Tbx2-3-4-5 in vertebrates during limb specification. Also, the fact that a Brachyury and a Tbx2 homologue exist in S. domuncula suggests that the first divergence from the ancestral Brachyury-like gene might be a Tbx2-like gene and not a Tbrain-like gene as had been previously suggested.
BACKGROUND INFORMATION: T-box transcription factors are a large family of transcriptional regulators involved in many aspects of embryonic development. In a previous report, we described the isolation and genomic characterization of two T-box genes from the siliceous sponge Suberites domuncula: a Brachyury homologue, Sd-Bra, and a Tbx2 homologue, Sd-Tbx2. Elucidation of the genomic structure of Sd-Bra allowed us to demonstrate the existence of two different isoforms, resulting from alternative splicing. Moreover, we demonstrated that the shorter isoform exists in two different glycosylation states. RESULTS: In the present study, we demonstrate a differential subcellular localization of the three Sd-Bra isoforms, suggesting that its differential nuclear import could be an important mechanism for its functional regulation. Furthermore, we demonstrate that Sd-Tbx2 exists only in one isoform, which is mainly localized in the nucleus. The pattern of expression of Sd-Bra and Sd-Tbx2 genes is analysed in sponge tissue, in gemmules and in cultured cells. CONCLUSION: These results suggest a conserved role for Sd-Bra in the control of morphogenetic movements through the regulation of cell-adhesion properties and the involvement of Sd-Tbx2 in the determination of cell identity in the early stages of differentiation, reminiscent of the function of Tbx2-3-4-5 in vertebrates during limb specification. Also, the fact that a Brachyury and a Tbx2 homologue exist in S. domuncula suggests that the first divergence from the ancestral Brachyury-like gene might be a Tbx2-like gene and not a Tbrain-like gene as had been previously suggested.
Authors: Kay Holstien; Ajna Rivera; Pam Windsor; Siyu Ding; Sally P Leys; Malcolm Hill; April Hill Journal: Dev Genes Evol Date: 2010-11-17 Impact factor: 0.900
Authors: Arnau Sebé-Pedrós; Alex de Mendoza; B Franz Lang; Bernard M Degnan; Iñaki Ruiz-Trillo Journal: Mol Biol Evol Date: 2010-11-17 Impact factor: 16.240
Authors: Amy C Horton; Navin R Mahadevan; Carolina Minguillon; Kazutoyo Osoegawa; Daniel S Rokhsar; Ilya Ruvinsky; Pieter J de Jong; Malcolm P Logan; Jeremy J Gibson-Brown Journal: Dev Genes Evol Date: 2008-09-25 Impact factor: 0.900