| Literature DB >> 15848121 |
Camillo Di Giulio1, Giuseppina Bianchi, Marisa Cacchio, Luciano Artese, Cinzia Rapino, Maria Antonietta Macrì, Carmine Di Ilio.
Abstract
To test if oxygen sensitive mechanisms are affected by hypoxia, we studied hypoxia inducible factor-1alpha (HIF-1alpha), vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) expression by immunohistochemical analysis in young and old rat carotid bodies (CBs) using hypoxia as a model for modulating aging. Four groups of male age-matched Wistar rats (3 and 24 months) were used. Two groups were kept in room air, and two groups were kept under chronic intermittent hypoxia for 12 days. In aged carotid body and in hypoxia the increased expression of HIF-1alpha, VEGF, iNOS is less evident as compared to the young one. Electron microscopy sections showed a reduced mitochondrial number and area in the aged CBs and during hypoxia. Less responsiveness to hypoxia could be evidenced in the aged rats as compared to the young rats, suggesting an age dependency of the oxygen sensitive mechanisms.Entities:
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Year: 2005 PMID： 15848121 DOI： 10.1016/j.resp.2005.01.006
Source DB: PubMed Journal: Respir Physiol Neurobiol ISSN： 1569-9048 Impact factor: 1.931