BACKGROUND: The platelet (PLT) alloimminization status after long-term red cell (RBC) transfusion in thalassemia patients was investigated, including antibodies against HLA antigens and PLT-specific glycoprotein antigens. STUDY DESIGN AND METHODS: Blood samples from a total of 60 thalassemia patients who routinely received washed RBCs were tested for the presence of HLA antibodies and PLT-specific glycoprotein antibodies with a commercial enzyme immunoassay kit. All patients were rescreened at a follow-up period of 12 to 15 months. RESULTS: At the first year of study, 19 (31%) patients had HLA antibodies, 13 (22%) had HLA antibodies and PLT-specific antibodies, and 1 (2%) had PLT-specific antibodies. One patient showed weak reactive PLT autoantibody. The follow-up study showed that 7 patients developed HLA antibodies, whereas 1 patient lost HLA antibody activity. Nine patients developed new PLT-specific antibodies, yet 12 patients lost at least one of their PLT-specific antibodies. CONCLUSION: Long-term RBC transfusions can induce PLT alloimmunization, both to HLA antigens and to PLT-specific antigens. The residual PLTs and white blood cells in RBC components could be the sources of immunization. In our thalassemia patients, HLA antibodies likely sustain longer than PLT-specific antibodies.
BACKGROUND: The platelet (PLT) alloimminization status after long-term red cell (RBC) transfusion in thalassemiapatients was investigated, including antibodies against HLA antigens and PLT-specific glycoprotein antigens. STUDY DESIGN AND METHODS: Blood samples from a total of 60 thalassemiapatients who routinely received washed RBCs were tested for the presence of HLA antibodies and PLT-specific glycoprotein antibodies with a commercial enzyme immunoassay kit. All patients were rescreened at a follow-up period of 12 to 15 months. RESULTS: At the first year of study, 19 (31%) patients had HLA antibodies, 13 (22%) had HLA antibodies and PLT-specific antibodies, and 1 (2%) had PLT-specific antibodies. One patient showed weak reactive PLT autoantibody. The follow-up study showed that 7 patients developed HLA antibodies, whereas 1 patient lost HLA antibody activity. Nine patients developed new PLT-specific antibodies, yet 12 patients lost at least one of their PLT-specific antibodies. CONCLUSION: Long-term RBC transfusions can induce PLT alloimmunization, both to HLA antigens and to PLT-specific antigens. The residual PLTs and white blood cells in RBC components could be the sources of immunization. In our thalassemiapatients, HLA antibodies likely sustain longer than PLT-specific antibodies.
Authors: Mohammad Hadi Sadeghian; Mohammad Reza Keramati; Zahra Badiei; Mehrangiz Ravarian; Hossein Ayatollahi; Houshang Rafatpanah; Mohammad Khajeh Daluei Journal: Asian J Transfus Sci Date: 2009-07
Authors: Marianne E McPherson; Alan R Anderson; Marta-Inés Castillejo; Christopher D Hillyer; Robert A Bray; Howard M Gebel; Cassandra D Josephson Journal: Pediatr Blood Cancer Date: 2010-04 Impact factor: 3.167