BACKGROUND: Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins. STUDY DESIGN AND METHODS: By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems. RESULTS: Unambiguous and concordant results were obtained with both techniques for all samples. CONCLUSION: The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.
BACKGROUND: Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins. STUDY DESIGN AND METHODS: By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems. RESULTS: Unambiguous and concordant results were obtained with both techniques for all samples. CONCLUSION: The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.
Authors: Cedric Ghevaert; David A Wilcox; Juan Fang; Kathryn L Armour; Mike R Clark; Willem H Ouwehand; Lorna M Williamson Journal: J Clin Invest Date: 2008-08 Impact factor: 14.808
Authors: Nicholas S Gleadall; Barbera Veldhuisen; Jeremy Gollub; Adam S Butterworth; John Ord; Christopher J Penkett; Tiffany C Timmer; Carolin M Sauer; Nieke van der Bolt; Colin Brown; Kim Brugger; Alexander T Dilthey; Daniel Duarte; Shane Grimsley; Katja van den Hurk; John M Jongerius; Jessie Luken; Karyn Megy; Gail Miflin; Christopher S Nelson; Femmeke J Prinsze; Jennifer Sambrook; Ilenia Simeoni; Michael Sweeting; Nicole Thornton; Sara Trompeter; Salih Tuna; Ram Varma; Matthew R Walker; John Danesh; David J Roberts; Willem H Ouwehand; Kathleen E Stirrups; Augusto Rendon; Connie M Westhoff; Emanuele Di Angelantonio; C Ellen van der Schoot; William J Astle; Nicholas A Watkins; William J Lane Journal: Blood Adv Date: 2020-08-11