Analytical strategies for residue analysis of veterinary drugs and growth-promoting agents in food-producing animals--a review.

After a brief introduction into the field of veterinary drugs and growth-promoting agents, the most important EU regulations and directives for the inspection of food-producing animals and animal products regarding the residue control of these substances are presented and discussed. Main attention in the review is on the methods of analysis in use today for the most important classes of veterinary drugs and growth-promoting agents viz. anthelmintics, antibiotics, coccidiostats, hormones, beta-agonists and tranquillizers. Emphasis is given to the potential, and limitations, of state-of-the-art analytical procedures and their performance characteristics. The most obvious conclusion is that, today (reversed-phase) liquid chromatography combined with tandem mass spectrometric detection--either triple-quadrupole or ion-trap multi-stage--is the preferred technique in a large majority of all cases. In the field of sample treatment, the combined use of liquid extraction--i.e., liquid partitioning or liquid-liquid extraction--and liquid-solid extraction--primary on- or off-line solid-phase extraction--is most popular. Finally, while the analytical tools required to meet the demands typically formulated by governments and international organizations today, generally speaking are available, several problems still do exist. To quote three examples, problems are encountered in the area of simultaneously extracting and pre-treating groups of analytes with mutually widely different polarities, with regard to identification-point--based confirmation of analyte identity, and regarding quantification errors caused by ion-suppression effects. Improving the speed of analysis is another aspect that should, and will, receive dedicated interest in the near future.