OBJECTIVES: To develop a reliable method to isolate fetal cells for genetic diagnosis. DESIGN: Aspiration of cervical mucus from pregnant women in the first trimester. SETTING: Pregnant women were recruited before an elective termination of pregnancy. POPULATION: Sixty pregnant women (7-10 weeks of gestation). METHODS: Fetal cells were isolated from aspirated cervical mucus of pregnant women using a combination of enzymatic digestion, fluorescent immunohistochemistry, micromanipulation and single-cell DNA allelic profiling. MAIN OUTCOME MEASURES: The isolation and identification of fetal cells. RESULTS: The transformation of the tenacious cervical mucus into a single-cell suspension enabled the isolation and identification of fetal cells by fluorescent immunohistochemistry. Confirmation of fetal origin was accomplished by single-cell DNA allelic profiling alongside known maternal cells. CONCLUSIONS: This novel non-invasive method is rapid and efficient with results attainable within 24 hours as early as seven weeks of gestation. The technique would offer earlier reassurance and the option of first trimester therapeutic abortions to both high and low risk pregnant women.
OBJECTIVES: To develop a reliable method to isolate fetal cells for genetic diagnosis. DESIGN: Aspiration of cervical mucus from pregnant women in the first trimester. SETTING: Pregnant women were recruited before an elective termination of pregnancy. POPULATION: Sixty pregnant women (7-10 weeks of gestation). METHODS: Fetal cells were isolated from aspirated cervical mucus of pregnant women using a combination of enzymatic digestion, fluorescent immunohistochemistry, micromanipulation and single-cell DNA allelic profiling. MAIN OUTCOME MEASURES: The isolation and identification of fetal cells. RESULTS: The transformation of the tenacious cervical mucus into a single-cell suspension enabled the isolation and identification of fetal cells by fluorescent immunohistochemistry. Confirmation of fetal origin was accomplished by single-cell DNA allelic profiling alongside known maternal cells. CONCLUSIONS: This novel non-invasive method is rapid and efficient with results attainable within 24 hours as early as seven weeks of gestation. The technique would offer earlier reassurance and the option of first trimester therapeutic abortions to both high and low risk pregnant women.
Authors: Kristin D Gerson; Miriam J Haviland; Dayna Neo; Jonathan L Hecht; Andrea A Baccarelli; Kasey Jm Brennan; Alexandra E Dereix; Steven J Ralston; Michele R Hacker; Heather H Burris Journal: Epigenomics Date: 2020-08-18 Impact factor: 4.778
Authors: Anthony N Imudia; Sanjeev Kumar; Michael P Diamond; Alan H DeCherney; D Randall Armant Journal: Fertil Steril Date: 2010-01-13 Impact factor: 7.329
Authors: Rani Fritz; Hamid Reza Kohan-Ghadr; Alex Sacher; Alan D Bolnick; Brian A Kilburn; Jay M Bolnick; Michael P Diamond; Sascha Drewlo; D Randall Armant Journal: Prenat Diagn Date: 2015-09-20 Impact factor: 3.050
Authors: Anthony N Imudia; Yoko Suzuki; Brian A Kilburn; Frank D Yelian; Michael P Diamond; Roberto Romero; D Randall Armant Journal: Hum Reprod Date: 2009-06-04 Impact factor: 6.918
Authors: Chandni V Jain; Leena Kadam; Marie van Dijk; Hamid-Reza Kohan-Ghadr; Brian A Kilburn; Craig Hartman; Vicki Mazzorana; Allerdien Visser; Michael Hertz; Alan D Bolnick; Rani Fritz; D Randall Armant; Sascha Drewlo Journal: Sci Transl Med Date: 2016-11-02 Impact factor: 19.319