Literature DB >> 1584067

A unique monoclonal antibody that recognizes mature p17 of HIV-1 but not its precursor.

A Saitoh1, N Tanaka, A Nakata, K Ikuta, H Shinagawa.   

Abstract

The entire and partial gag regions of human immunodeficiency virus type 1 (HIV-1) were overproduced in Escherichia coli and used for epitope mapping of antibodies against p17. We found that a mouse monoclonal antibody to p17, V17 recognizes the mature p17 but not the unprocessed Gag proteins containing the entire p17 moiety. Further analysis revealed that V17 recognizes the C-terminal 12-amino-acid region of p17 having free C-terminus. This monoclonal antibody may be useful for monitoring the maturation of virus particles.

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Year:  1992        PMID： 1584067     DOI： 10.1111/j.1348-0421.1992.tb01647.x

Source DB:  PubMed          Journal:  Microbiol Immunol        ISSN： 0385-5600            Impact factor:   1.955


  5 in total

1.  Optimal conditions of immune complex transfer enzyme immunoassays for antibody IgGs to HIV-1 using recombinant p17, p24, and reverse transcriptase as antigens.

Authors:  S Hashida; S Ishikawa; K Hashinaka; I Nishikata; S Oka; K Shimada; A Saito; A Takamizawa; H Shinagawa; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

2.  More sensitive immune complex transfer enzyme immunoassay for antibody IgG to p17 of HIV-1 with shorter incubation time for immunoreactions and larger volumes of serum samples.

Authors:  S Ishikawa; S Hashida; K Hashinaka; M Kojima; A Saito; A Takamizawa; H Shinagawa; S Oka; K Shimada; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1997       Impact factor: 2.352

Review 3.  More reliable diagnosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, and/or serum with highly sensitive and specific enzyme immunoassay (immune complex transfer enzyme immunoassay): a review.

Authors:  S Hashida; K Hashinaka; S Ishikawa; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1997       Impact factor: 2.352

4.  Earlier detection of human immunodeficiency virus type 1 p24 antigen and immunoglobulin G and M antibodies to p17 antigen in seroconversion serum panels by immune complex transfer enzyme immunoassays.

Authors:  S Hashida; S Ishikawa; K Hashinaka; I Nishikata; S Oka; E Ishikawa
Journal:  Clin Diagn Lab Immunol       Date:  2000-11

5.  Immune complex transfer enzyme immunoassay that is more sensitive and specific than western blotting for detection of antibody immunoglobulin G to human immunodeficiency virus type 1 in serum with recombinant pol and gag proteins as antigens.

Authors:  S Hashida; K Hashinaka; I Nishikata; S Oka; K Shimada; A Saito; A Takamizawa; H Shinagawa; S Yano; H Kojima
Journal:  Clin Diagn Lab Immunol       Date:  1995-09
  5 in total

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