| Literature DB >> 15837562 |
Masayuki Morikawa1, John D Fryer, Patrick M Sullivan, Erin A Christopher, Suzanne E Wahrle, Ronald B DeMattos, Mark A O'Dell, Anne M Fagan, Hilal A Lashuel, Thomas Walz, Kiyofumi Asai, David M Holtzman.
Abstract
The apolipoprotein E (apoE) genotype is an important genetic risk factor for Alzheimer's disease (AD). In the central nervous system (CNS), most apoE is produced by astrocytes and is present in unique high-density lipoprotein (HDL)-like particles that have distinct properties from apoE derived from other sources. To develop an efficient system to produce astrocyte-derived apoE in large quantities, we produced and characterized immortalized cell lines from primary astrocyte cultures derived from human APOE knock-in mice. APOE2, APOE3, and APOE4 expressing cell lines were established that secrete apoE in HDL-like particles at similar levels, cholesterol composition, and size as those produced by primary astrocytes. In physiological buffers, astrocyte-secreted apoE3 and E4 associated equally well with amyloid-beta. Under the same conditions, only a small fraction of A beta formed sodium dodecyl sulfate (SDS)-stable complexes with apoE (E3 > E4). These immortalized astrocytes will be useful for studying mechanisms underlying the isoform-specific effects of apoE in the CNS.Entities:
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Year: 2005 PMID： 15837562 DOI： 10.1016/j.nbd.2004.11.005
Source DB: PubMed Journal: Neurobiol Dis ISSN： 0969-9961 Impact factor: 5.996