Zhong-Jing Su1, Hai-Bin Chen, Jin-Kun Zhang, Lan Xu. 1. Cancer Pathology Laboratory, Department of Histology and Embryology, Shantou University Medical College, Shantou 515041, Guangdong Province, China.
Abstract
AIM: To develop a cancer vaccine of dendritic cells derived from human cord blood CD34+ cells and to investigate its cytotoxicity on human hepatocarcinoma cells in vitro and in severe combined immunodeficiency (SCID) mice. METHODS: Lymphocytes from cord blood or peripheral blood were primed by DCs, which were derived from cord blood and pulsed with whole tumor cell lysates. Nonradiative neutral red uptake assay was adopted to detect the cytotoxicity of primed lymphocytes on human hepatocarcinoma cell line BEL-7402 in vitro. The anti-tumor effect of primed lymphocytes in vivo was detected in SCID mice, including therapeutic effect and vaccination effect. RESULTS: The cytotoxicity of DC vaccine primed lymphocytes from cord blood or peripheral blood on human hepatocarcinoma cell line BEL-7402 was significantly higher than that of unprimed lymphocytes in vitro (44.09% vs 14.69%, 47.92% vs 19.44%, P<0.01). There was no significant difference between the cytotoxicity of primed lymphocytes from cord blood and peripheral blood (P>0.05). The tumor growth rate and tumor size were smaller in SCID mice treated or vaccinated with primed lymphocytes than those with unprimed lymphocytes. SCID mice vaccinated with primed lymphocytes had a lower tumor incidence (80% vs 100%, P<0.05) and delayed tumor latent period compared with mice vaccinated with unprimed lymphocytes (11 d vs 7 d, P<0.01). CONCLUSION: Vaccine of cord blood derived-DCs has an inhibitory activity on growth of human hepatocarcinoma cells in vitro and in SCID mice. The results also implicate the potential role of cord blood derived-DC vaccine in clinical tumor immunotherapy.
AIM: To develop a cancer vaccine of dendritic cells derived from human cord blood CD34+ cells and to investigate its cytotoxicity on human hepatocarcinoma cells in vitro and in severe combined immunodeficiency (SCID) mice. METHODS: Lymphocytes from cord blood or peripheral blood were primed by DCs, which were derived from cord blood and pulsed with whole tumor cell lysates. Nonradiative neutral red uptake assay was adopted to detect the cytotoxicity of primed lymphocytes on human hepatocarcinoma cell line BEL-7402 in vitro. The anti-tumor effect of primed lymphocytes in vivo was detected in SCIDmice, including therapeutic effect and vaccination effect. RESULTS: The cytotoxicity of DC vaccine primed lymphocytes from cord blood or peripheral blood on human hepatocarcinoma cell line BEL-7402 was significantly higher than that of unprimed lymphocytes in vitro (44.09% vs 14.69%, 47.92% vs 19.44%, P<0.01). There was no significant difference between the cytotoxicity of primed lymphocytes from cord blood and peripheral blood (P>0.05). The tumor growth rate and tumor size were smaller in SCIDmice treated or vaccinated with primed lymphocytes than those with unprimed lymphocytes. SCIDmice vaccinated with primed lymphocytes had a lower tumor incidence (80% vs 100%, P<0.05) and delayed tumor latent period compared with mice vaccinated with unprimed lymphocytes (11 d vs 7 d, P<0.01). CONCLUSION: Vaccine of cord blood derived-DCs has an inhibitory activity on growth of human hepatocarcinoma cells in vitro and in SCIDmice. The results also implicate the potential role of cord blood derived-DC vaccine in clinical tumor immunotherapy.
Authors: T B Geijtenbeek; R Torensma; S J van Vliet; G C van Duijnhoven; G J Adema; Y van Kooyk; C G Figdor Journal: Cell Date: 2000-03-03 Impact factor: 41.582
Authors: C Caux; C Massacrier; C Dezutter-Dambuyant; B Vanbervliet; C Jacquet; D Schmitt; J Banchereau Journal: J Immunol Date: 1995-12-01 Impact factor: 5.422
Authors: C Caux; B Vanbervliet; C Massacrier; C Dezutter-Dambuyant; B de Saint-Vis; C Jacquet; K Yoneda; S Imamura; D Schmitt; J Banchereau Journal: J Exp Med Date: 1996-08-01 Impact factor: 14.307
Authors: Ludovic Tailleux; Olivier Schwartz; Jean-Louis Herrmann; Elisabeth Pivert; Mary Jackson; Ali Amara; Luc Legres; Donatus Dreher; Laurent P Nicod; Jean Claude Gluckman; Philippe H Lagrange; Brigitte Gicquel; Olivier Neyrolles Journal: J Exp Med Date: 2003-01-06 Impact factor: 14.307