Literature DB >> 15831961

RNA interference of rotavirus segment 11 mRNA reveals the essential role of NSP5 in the virus replicative cycle.

Michela Campagna1, Catherine Eichwald1, Fulvia Vascotto1, Oscar R Burrone1.   

Abstract

Rotavirus genomes contain 11 double-stranded (ds) RNA segments. Genome segment 11 encodes the non-structural protein NSP5 and, in some strains, also NSP6. NSP5 is produced soon after viral infection and localizes in cytoplasmic viroplasms, where virus replication takes place. RNA interference by small interfering (si) RNAs targeted to genome segment 11 mRNA of two different strains blocked production of NSP5 in a strain-specific manner, with a strong effect on the overall replicative cycle: inhibition of viroplasm formation, decreased production of other structural and non-structural proteins, synthesis of viral genomic dsRNA and production of infectious particles. These effects were shown not to be due to inhibition of NSP6. The results obtained strengthen the importance of secondary transcription/translation in rotavirus replication and demonstrate that NSP5 is essential for the assembly of viroplasms and virus replication.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 15831961     DOI： 10.1099/vir.0.80598-0

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  43 in total

1.  Gene-specific inhibition of reovirus replication by RNA interference.

Authors:  Takeshi Kobayashi; James D Chappell; Pranav Danthi; Terence S Dermody
Journal:  J Virol       Date:  2006-09       Impact factor: 5.103

2.  Rotavirus Nonstructural Protein NSP3 is not required for viral protein synthesis.

Authors:  Hilda Montero; Carlos F Arias; Susana Lopez
Journal:  J Virol       Date:  2006-09       Impact factor: 5.103

3.  Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication.

Authors:  Winsome Cheung; Michael Gill; Alessandro Esposito; Clemens F Kaminski; Nathalie Courousse; Serge Chwetzoff; Germain Trugnan; Nandita Keshavan; Andrew Lever; Ulrich Desselberger
Journal:  J Virol       Date:  2010-03-24       Impact factor: 5.103

4.  Interaction of rotavirus polymerase VP1 with nonstructural protein NSP5 is stronger than that with NSP2.

Authors:  F Arnoldi; M Campagna; C Eichwald; U Desselberger; O R Burrone
Journal:  J Virol       Date:  2006-12-20       Impact factor: 5.103

5.  Hyperphosphorylation of the rotavirus NSP5 protein is independent of serine 67, [corrected] NSP2, or [corrected] the intrinsic insolubility of NSP5 is regulated by cellular phosphatases.

Authors:  Adrish Sen; Darin Agresti; Erich R Mackow
Journal:  J Virol       Date:  2006-02       Impact factor: 5.103

6.  Cryoelectron microscopy structures of rotavirus NSP2-NSP5 and NSP2-RNA complexes: implications for genome replication.

Authors:  Xiaofang Jiang; Hariharan Jayaram; Mukesh Kumar; Steven J Ludtke; Mary K Estes; B V Venkataram Prasad
Journal:  J Virol       Date:  2006-08-23       Impact factor: 5.103

7.  Silencing and complementation of reovirus core protein mu2: functional correlations with mu2-microtubule association and differences between virus- and plasmid-derived mu2.

Authors:  John Carvalho; Michelle M Arnold; Max L Nibert
Journal:  Virology       Date:  2007-04-23       Impact factor: 3.616

8.  The formation of viroplasm-like structures by the rotavirus NSP5 protein is calcium regulated and directed by a C-terminal helical domain.

Authors:  Adrish Sen; Nandini Sen; Erich R Mackow
Journal:  J Virol       Date:  2007-08-15       Impact factor: 5.103

9.  A novel form of rotavirus NSP2 and phosphorylation-dependent NSP2-NSP5 interactions are associated with viroplasm assembly.

Authors:  Jeanette M Criglar; Liya Hu; Sue E Crawford; Joseph M Hyser; James R Broughman; B V Venkataram Prasad; Mary K Estes
Journal:  J Virol       Date:  2013-11-06       Impact factor: 5.103

10.  Sequestration of free tubulin molecules by the viral protein NSP2 induces microtubule depolymerization during rotavirus infection.

Authors:  Davy Martin; Mariela Duarte; Jean Lepault; Didier Poncet
Journal:  J Virol       Date:  2009-12-23       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.