Effect of amphotericin B and clotrimazole on lymphocyte stimulation.

Human lymphocytes were stimulated in vitro by phytohemagglutinin, concanavalin A, pokeweed mitogen, purified protein derivative-tuberculin, and allogenic cells. The deoxyribonucleic acid synthesis of the lymphocytes was inhibited in increasing degree by 4 to 8 mug of amphotericin B per ml of culture irrespective of lymphocyte stimulant used. The effect of clotrimazole on the deoxyribonucleic acid synthesis varied between experiments with different and also between experiments with the same stimulant. Ten micrograms of clotrimazole per ml was generally inhibiting, whereas in one experiment 2 mug or more per ml inhibited the purified protein derivative-induced deoxyribonucleic acid synthesis. The effects of amphotericin B and clotrimazole were neutralized by serum.