OBJECTIVE: To relate levels of vascular endothelial growth factor (VEGF) and its soluble receptor, sFlt-1, with endothelial function in healthy smokers. METHODS: Plasma levels of VEGF and sFlt-1 were measured by ELISA in 22 healthy smokers and 22 matched healthy non-smoking controls, and compared to flow- (FMD) and acetylcholine-mediated (AMD) vasodilatation (endothelial-dependent) (EDV) and nitroglycerine-mediated (NMD) vasodilatation (endothelial-independent) of lower extremities were measured with plethysmography. RESULTS: Smokers and controls had similar plasma VEGF levels, but sFlt-1 levels were lower in smokers than in controls (p<0.01). AMD was lower in smokers compared to controls (p<0.05), but FMD and NMD levels were similar. Smokers and controls with high AMD (>12 ml/100 ml tissue/min) had significantly lower plasma VEGF levels (p<0.001). An inverse correlation was found in both groups, between VEGF and AMD (smokers: r=-0.6, p<0.01; controls: r=-0.71, p<0.005) and with FMD (smokers: r=-0.56, p<0.05; controls: r=-0.58, p<0.005). There were no significant correlations between sFlt-1 with VEGF levels or endothelial-dependent dilatation. CONCLUSION: In conclusion, healthy smokers demonstrate abnormal AMD, and an inverse correlation between plasma VEGF levels (but not sFlt-1) with indices of endothelial dysfunction (FMD and AMD) exists. VEGF, and not sFlt-1, may be related to the pathogenesis of endothelial dysfunction in healthy smoking individuals.
OBJECTIVE: To relate levels of vascular endothelial growth factor (VEGF) and its soluble receptor, sFlt-1, with endothelial function in healthy smokers. METHODS: Plasma levels of VEGF and sFlt-1 were measured by ELISA in 22 healthy smokers and 22 matched healthy non-smoking controls, and compared to flow- (FMD) and acetylcholine-mediated (AMD) vasodilatation (endothelial-dependent) (EDV) and nitroglycerine-mediated (NMD) vasodilatation (endothelial-independent) of lower extremities were measured with plethysmography. RESULTS: Smokers and controls had similar plasma VEGF levels, but sFlt-1 levels were lower in smokers than in controls (p<0.01). AMD was lower in smokers compared to controls (p<0.05), but FMD and NMD levels were similar. Smokers and controls with high AMD (>12 ml/100 ml tissue/min) had significantly lower plasma VEGF levels (p<0.001). An inverse correlation was found in both groups, between VEGF and AMD (smokers: r=-0.6, p<0.01; controls: r=-0.71, p<0.005) and with FMD (smokers: r=-0.56, p<0.05; controls: r=-0.58, p<0.005). There were no significant correlations between sFlt-1 with VEGF levels or endothelial-dependent dilatation. CONCLUSION: In conclusion, healthy smokers demonstrate abnormal AMD, and an inverse correlation between plasma VEGF levels (but not sFlt-1) with indices of endothelial dysfunction (FMD and AMD) exists. VEGF, and not sFlt-1, may be related to the pathogenesis of endothelial dysfunction in healthy smoking individuals.
Authors: Arun Jeyabalan; Robert W Powers; Allison R Durica; Gail F Harger; James M Roberts; Roberta B Ness Journal: Am J Hypertens Date: 2008-06-19 Impact factor: 2.689
Authors: Arun Jeyabalan; Robert W Powers; Rebecca G Clifton; Peter Van Dorsten; John C Hauth; Mark A Klebanoff; Marshall D Lindheimer; Baha Sibai; Mark Landon; Menachem Miodovnik Journal: PLoS One Date: 2010-10-12 Impact factor: 3.240
Authors: Wolfgang Lieb; Radwan Safa; Emelia J Benjamin; Vanessa Xanthakis; Xiaoyan Yin; Lisa M Sullivan; Martin G Larson; Holly M Smith; Joseph A Vita; Gary F Mitchell; Douglas B Sawyer; Ramachandran S Vasan Journal: Eur Heart J Date: 2009-02-17 Impact factor: 29.983
Authors: Hironori Uehara; Christina Mamalis; Molly McFadden; Michael Taggart; Brian Stagg; Samuel Passi; Phillip Earle; Usha Chakravarthy; Ruth E Hogg; Balamurali K Ambati Journal: Am J Ophthalmol Date: 2014-10-18 Impact factor: 5.258
Authors: Farzad Moazed; Ellen L Burnham; R William Vandivier; Cecilia M O'Kane; Murali Shyamsundar; Umar Hamid; Jason Abbott; David R Thickett; Michael A Matthay; Daniel F McAuley; Carolyn S Calfee Journal: Thorax Date: 2016-02-02 Impact factor: 9.139
Authors: Amy Webb; Audrey C Papp; Amanda Curtis; Leslie C Newman; Maciej Pietrzak; Michal Seweryn; Samuel K Handelman; Grzegorz A Rempala; Daqing Wang; Erica Graziosa; Rachel F Tyndale; Caryn Lerman; John R Kelsoe; Deborah C Mash; Wolfgang Sadee Journal: BMC Genomics Date: 2015-11-23 Impact factor: 3.969
Authors: Kameshwar P Singh; Gina Lawyer; Thivanka Muthumalage; Krishna P Maremanda; Naushad Ahmad Khan; Samantha R McDonough; Dongxia Ye; Scott McIntosh; Irfan Rahman Journal: ERJ Open Res Date: 2019-12-23