| Literature DB >> 15823401 |
Alena Jirásková1, Libor Vítek, Johan Fevery, Tomás Ruml, Pavel Branny.
Abstract
A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.Entities:
Mesh：
Year: 2005 PMID： 15823401 DOI： 10.1016/j.mimet.2005.01.012
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363