| Literature DB >> 1582182 |
A E Goodwin1, J M Grizzle, J T Bradley, B H Estridge.
Abstract
1. A monoclonal antibody to vitellogenin of channel catfish (Ictalurus punctatus) was made, and its specificity was demonstrated using Western blots of serum from female fish, estradiol-treated male fish, untreated male fish, vitellogenin purified by three different methods and egg extracts. 2. An enzyme-linked immunosorbent assay (ELISA), using this monoclonal antibody, detected vitellogenin in the plasma of 59 out of 60 untreated 17-24-month-old male channel catfish with a mean concentration of 338 micrograms/ml and a maximum concentration of 4240 micrograms/ml. 3. Vitellogenin levels in male channel catfish were unrelated to testicular stage, gonadosomatic index and month.Entities:
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Year: 1992 PMID： 1582182 DOI： 10.1016/0305-0491(92)90025-m
Source DB: PubMed Journal: Comp Biochem Physiol B ISSN： 0305-0491