Literature DB >> 15820980

Computationally designed variants of Escherichia coli chorismate mutase show altered catalytic activity.

Jonathan Kyle Lassila1, Jennifer R Keeffe, Peter Oelschlaeger, Stephen L Mayo.   

Abstract

Computational protein design methods were used to predict five variants of monofunctional Escherichia coli chorismate mutase expected to maintain catalytic activity. The variants were tested experimentally and three active site mutants exhibited catalytic activity similar to or greater than the wild-type enzyme. One mutant, Ala32Ser, showed increased catalytic efficiency.

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Year:  2005        PMID： 15820980     DOI： 10.1093/protein/gzi015

Source DB:  PubMed          Journal:  Protein Eng Des Sel        ISSN： 1741-0126            Impact factor:   1.650


  10 in total

1.  Improving computational protein design by using structure-derived sequence profile.

Authors:  Liang Dai; Yuedong Yang; Hyung Rae Kim; Yaoqi Zhou
Journal:  Proteins       Date:  2010-08-01

2.  Combinatorial methods for small-molecule placement in computational enzyme design.

Authors:  Jonathan Kyle Lassila; Heidi K Privett; Benjamin D Allen; Stephen L Mayo
Journal:  Proc Natl Acad Sci U S A       Date:  2006-10-30       Impact factor: 11.205

Review 3.  Progress in computational protein design.

Authors:  Shaun M Lippow; Bruce Tidor
Journal:  Curr Opin Biotechnol       Date:  2007-07-20       Impact factor: 9.740

Review 4.  Challenges in the computational design of proteins.

Authors:  María Suárez; Alfonso Jaramillo
Journal:  J R Soc Interface       Date:  2009-03-11       Impact factor: 4.118

5.  Expanded explorations into the optimization of an energy function for protein design.

Authors:  Yao-Ming Huang; Christopher Bystroff
Journal:  IEEE/ACM Trans Comput Biol Bioinform       Date:  2013 Sep-Oct       Impact factor: 3.710

Review 6.  Energy functions in de novo protein design: current challenges and future prospects.

Authors:  Zhixiu Li; Yuedong Yang; Jian Zhan; Liang Dai; Yaoqi Zhou
Journal:  Annu Rev Biophys       Date:  2013-02-28       Impact factor: 12.981

7.  Computational design of antibody-affinity improvement beyond in vivo maturation.

Authors:  Shaun M Lippow; K Dane Wittrup; Bruce Tidor
Journal:  Nat Biotechnol       Date:  2007-09-23       Impact factor: 54.908

8.  The two chorismate mutases from both Mycobacterium tuberculosis and Mycobacterium smegmatis: biochemical analysis and limited regulation of promoter activity by aromatic amino acids.

Authors:  Cristopher Z Schneider; Tanya Parish; Luiz A Basso; Diógenes S Santos
Journal:  J Bacteriol       Date:  2007-10-26       Impact factor: 3.490

9.  Directed evolution strategies for improved enzymatic performance.

Authors:  Edward G Hibbert; Paul A Dalby
Journal:  Microb Cell Fact       Date:  2005-10-07       Impact factor: 5.328

Review 10.  Strategies for discovery and improvement of enzyme function: state of the art and opportunities.

Authors:  Praveen Kaul; Yasuhisa Asano
Journal:  Microb Biotechnol       Date:  2011-08-24       Impact factor: 5.813
  10 in total

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