OBJECTIVE: To evaluate the influence of aging on testicular histology and the aneuploidy rate in testis postmeiotic cells. DESIGN: Comparison between older men and younger men. SETTING: Deceased donors and patients who requested assisted reproductive technology (ART). PATIENT(S): Thirty-six older men (61-102 years old) and 10 younger men (29-40 years old). INTERVENTION(S): Testes were obtained from 35 deceased donors, and testicular biopsies were obtained from 11 patients who requested ART. MAIN OUTCOME MEASURE(S): Histomorphometry of testis and fluorescent in situ hybridization (FISH), with a three-probe set X, Y, and 18. RESULT(S): The histomorphometric study showed a thickening of the basal membrane when spermatogenesis was arrested. The number of germinal and Sertoli cells decreased as age increased. The rate of aneuploidy of postmeiotic cells was 1.1% for the control group, 1.29% for older subjects with preserved spermatogenesis, and 14.28% for the subjects with an arrested spermiogenesis. Only this last figure was higher than the control group. CONCLUSION(S): The rate of aneuploidy in older subjects (61-95 years old) with preserved spermatogenesis was not statistically different from that found in the control group; it was increased in older subjects with arrested spermatogenesis.
OBJECTIVE: To evaluate the influence of aging on testicular histology and the aneuploidy rate in testis postmeiotic cells. DESIGN: Comparison between older men and younger men. SETTING: Deceased donors and patients who requested assisted reproductive technology (ART). PATIENT(S): Thirty-six older men (61-102 years old) and 10 younger men (29-40 years old). INTERVENTION(S): Testes were obtained from 35 deceased donors, and testicular biopsies were obtained from 11 patients who requested ART. MAIN OUTCOME MEASURE(S): Histomorphometry of testis and fluorescent in situ hybridization (FISH), with a three-probe set X, Y, and 18. RESULT(S): The histomorphometric study showed a thickening of the basal membrane when spermatogenesis was arrested. The number of germinal and Sertoli cells decreased as age increased. The rate of aneuploidy of postmeiotic cells was 1.1% for the control group, 1.29% for older subjects with preserved spermatogenesis, and 14.28% for the subjects with an arrested spermiogenesis. Only this last figure was higher than the control group. CONCLUSION(S): The rate of aneuploidy in older subjects (61-95 years old) with preserved spermatogenesis was not statistically different from that found in the control group; it was increased in older subjects with arrested spermatogenesis.
Authors: Xichen Nie; Sarah K Munyoki; Meena Sukhwani; Nina Schmid; Annika Missel; Benjamin R Emery; Jan-Bernd Stukenborg; Artur Mayerhofer; Kyle E Orwig; Kenneth I Aston; James M Hotaling; Bradley R Cairns; Jingtao Guo Journal: Dev Cell Date: 2022-05-02 Impact factor: 13.417
Authors: Denise R Archambeault; Jessica Tomaszewski; Andrew J Childs; Richard A Anderson; Humphrey Hung-Chang Yao Journal: Endocrinology Date: 2011-09-27 Impact factor: 4.736