AIM: To investigate the occurrence of cellular src (c-src) activating mutation at codon 531 in colorectal cancer patients from Chinese mainland. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay followed by sequencing and single-strand conformation polymor-phism analysis were carried out to screen 110 samples of primary colorectal cancer and 20 colorectal liver metastases. RESULTS: Only one sample showed PCR-RFLP-positive results and carried somatic codon 531 mutations. No additional mutation of c-src exon 12 was found. CONCLUSION: c-src codon 531 mutation in colorectal cancer is not the cause of c-src activation.
AIM: To investigate the occurrence of cellular src (c-src) activating mutation at codon 531 in colorectal cancerpatients from Chinese mainland. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay followed by sequencing and single-strand conformation polymor-phism analysis were carried out to screen 110 samples of primary colorectal cancer and 20 colorectal liver metastases. RESULTS: Only one sample showed PCR-RFLP-positive results and carried somatic codon 531 mutations. No additional mutation of c-src exon 12 was found. CONCLUSION:c-src codon 531 mutation in colorectal cancer is not the cause of c-src activation.
Authors: W R Cam; T Masaki; Y Shiratori; N Kato; T Ikenoue; M Okamoto; K Igarashi; T Sano; M Omata Journal: Cancer Date: 2001-07-01 Impact factor: 6.860
Authors: R B Irby; W Mao; D Coppola; J Kang; J M Loubeau; W Trudeau; R Karl; D J Fujita; R Jove; T J Yeatman Journal: Nat Genet Date: 1999-02 Impact factor: 38.330
Authors: H Wang; B Liu; A Q O Al-Aidaroos; H Shi; L Li; K Guo; J Li; B C P Tan; J M Loo; J P Tang; M Thura; Q Zeng Journal: Oncogene Date: 2015-10-12 Impact factor: 9.867