| Literature DB >> 15812312 |
Min Ye1, Shengdi Chen, Xijin Wang, Chen Qi, Guoqiang Lu, Liang Liang, Jieyi Xu.
Abstract
To investigate the capability of Sprague-Dawley rat bone marrow stromal cells to secrete glial cell line-derived neurotrophic factors (GDNF), we detected expression of GDNF messenger RNA and protein in bone marrow stromal cells of Sprague-Dawley rats by reverse-transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA), respectively. The GDNF messenger RNA and protein were detected in culture medium and total cell protein when bone marrow stromal cells were cultured for 3 days. The levels of GDNF in culture medium and total cell protein increased gradually after 3, 7 and 10 days of culture. Rat bone marrow stromal cells have the potential to secrete GDNF. Furthermore, the ability of secretion is determined by the surrounding microenvironment and self-growth status.Entities:
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Year: 2005 PMID： 15812312 DOI： 10.1097/00001756-200504250-00013
Source DB: PubMed Journal: Neuroreport ISSN： 0959-4965 Impact factor: 1.837