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Literature DB >> 15811807

High-throughput screens and selections of enzyme-encoding genes.

Amir Aharoni¹, Andrew D Griffiths, Dan S Tawfik.

Abstract

The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>10(6) gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

Mesh：

Substances：
Enzymes

Year: 2005 PMID： 15811807 DOI： 10.1016/j.cbpa.2005.02.002

Source DB: PubMed Journal: Curr Opin Chem Biol ISSN： 1367-5931 Impact factor: 8.822

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Cited

34 in total

1. Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility.

Authors: Lyad Zamir; Marianna Zaretsky; Yearit Fridman; Hadas Ner-Gaon; Eitan Rubin; Amir Aharoni
Journal: Proc Natl Acad Sci U S A Date: 2012-01-17 Impact factor: 11.205

High-throughput screens and selections of enzyme-encoding genes.

1. Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility.

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