| Literature DB >> 15811329 |
Tohru Tsuchiya1, Shinichi Takaichi, Norihiko Misawa, Takashi Maoka, Hideaki Miyashita, Mamoru Mimuro.
Abstract
Carotenoid composition and its biosynthetic pathway in the cyanobacterium Gloeobacter violaceus PCC 7421 were investigated. beta-Carotene and (2S,2'S)-oscillol 2,2'-di(alpha-L-fucoside), and echinenone were major and minor carotenoids, respectively. We identified two unique genes for carotenoid biosynthesis using in vivo functional complementation experiments. In Gloeobacter, a bacterial-type phytoene desaturase (CrtI), rather than plant-type desaturases (CrtP and CrtQ), produced lycopene. This is the first demonstration of an oxygenic photosynthetic organism utilizing bacterial-type phytoene desaturase. We also revealed that echinenone synthesis is catalyzed by CrtW rather than CrtO. These findings indicated that Gloeobacter retains ancestral properties of carotenoid biosynthesis.Entities:
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Year: 2005 PMID： 15811329 DOI： 10.1016/j.febslet.2005.02.066
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124