Tao Zhang1, Ping Li. 1. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Abstract
OBJECTIVE: To observe the expression of mRNA for the MIP-1alpha in cerebral tissue after hypoxic-ischemic brain damage (HIBD) in newborn rat. METHODS: The dynamic changes of expression of MIP-1alpha mRNA were studied using 7 d-old SD rat hypoxic-ischemic brain damage model and quantitative, real-time, fluorogenic PCR (TaqMan) assay. RESULTS: Peaking transcription was shown at 6 hours after HIBD when MIP-1alpha mRNA level in the HI cerebral hemisphere was almost 10 times compared with that of control group. The mRNA level of 12 and 24 hours after HIBD was also greater than that of control group. CONCLUSION: This appearance suggests that, as an inflammatory mediator, the MIP-1alpha transcription after HIBD may play important roles in the evolvement of brain damage.
OBJECTIVE: To observe the expression of mRNA for the MIP-1alpha in cerebral tissue after hypoxic-ischemic brain damage (HIBD) in newborn rat. METHODS: The dynamic changes of expression of MIP-1alpha mRNA were studied using 7 d-old SD rathypoxic-ischemic brain damage model and quantitative, real-time, fluorogenic PCR (TaqMan) assay. RESULTS: Peaking transcription was shown at 6 hours after HIBD when MIP-1alpha mRNA level in the HI cerebral hemisphere was almost 10 times compared with that of control group. The mRNA level of 12 and 24 hours after HIBD was also greater than that of control group. CONCLUSION: This appearance suggests that, as an inflammatory mediator, the MIP-1alpha transcription after HIBD may play important roles in the evolvement of brain damage.
Authors: Ubaldo E Martinez-Outschoorn; Diana Whitaker-Menezes; Zhao Lin; Neal Flomenberg; Anthony Howell; Richard G Pestell; Michael P Lisanti; Federica Sotgia Journal: Cell Cycle Date: 2011-06-01 Impact factor: 4.534
Authors: Constantino Tomas-Sanchez; Victor Manuel Blanco-Alvarez; Juan Antonio Gonzalez-Barrios; Daniel Martinez-Fong; Guadalupe Garcia-Robles; Guadalupe Soto-Rodriguez; Eduardo Brambila; Maricela Torres-Soto; Alejandro Gonzalez-Vazquez; Ana Karina Aguilar-Peralta; José-Luis Garate-Morales; Luis-Angel Aguilar-Carrasco; Daniel I Limón; Jorge Cebada; Bertha Alicia Leon-Chavez Journal: J Immunol Res Date: 2016-08-18 Impact factor: 4.818