M Tarek Elghetany1, Bruce H Davis. 1. Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0743, USA. melgheta@utmb.edu
Abstract
BACKGROUND: There is a gradual but steady increase in the use of granulocytic surface marker studies to diagnose several inherited and acquired blood and bone marrow disorders. Diagnosis and follow-up of patients with inflammation and infection are other areas of quantitative flow cytometric application. Despite the increased use of flow cytometry to study granulocytes, there seems to be no well-established standards regarding specimen handling for these studies. METHODS: This review summarizes the effect of preanalytical variables on granulocytic surface markers. RESULTS AND CONCLUSIONS: Storing specimens in sodium heparin at room temperature for up to 72 h seems satisfactory. Other anticoagulants, although acceptable, may have a shorter storage time. Storage time could be prolonged further with the use of some preservation media. Lysed whole blood is the preferred technique. Techniques should avoid major temperature change and excessive manipulation and should maintain storage and methodologic temperature with minimal fluctuation. Fixation before staining with the antibody may result in decreased expression of some surface antigens. Copyright 2005 Wiley-Liss, Inc.
BACKGROUND: There is a gradual but steady increase in the use of granulocytic surface marker studies to diagnose several inherited and acquired blood and bone marrow disorders. Diagnosis and follow-up of patients with inflammation and infection are other areas of quantitative flow cytometric application. Despite the increased use of flow cytometry to study granulocytes, there seems to be no well-established standards regarding specimen handling for these studies. METHODS: This review summarizes the effect of preanalytical variables on granulocytic surface markers. RESULTS AND CONCLUSIONS: Storing specimens in sodium heparin at room temperature for up to 72 h seems satisfactory. Other anticoagulants, although acceptable, may have a shorter storage time. Storage time could be prolonged further with the use of some preservation media. Lysed whole blood is the preferred technique. Techniques should avoid major temperature change and excessive manipulation and should maintain storage and methodologic temperature with minimal fluctuation. Fixation before staining with the antibody may result in decreased expression of some surface antigens. Copyright 2005 Wiley-Liss, Inc.
Authors: Arjan A van de Loosdrecht; Canan Alhan; Marie Christine Béné; Matteo G Della Porta; Angelika M Dräger; Jean Feuillard; Patricia Font; Ulrich Germing; Detlef Haase; Christa H Homburg; Robin Ireland; Joop H Jansen; Wolfgang Kern; Luca Malcovati; Jeroen G Te Marvelde; Ghulam J Mufti; Kiyoyuki Ogata; Alberto Orfao; Gert J Ossenkoppele; Anna Porwit; Frank W Preijers; Stephen J Richards; Gerrit Jan Schuurhuis; Dolores Subirá; Peter Valent; Vincent H J van der Velden; Paresh Vyas; August H Westra; Theo M de Witte; Denise A Wells; Michael R Loken; Theresia M Westers Journal: Haematologica Date: 2009-06-22 Impact factor: 9.941
Authors: Gizem Turaç; Christopher J Hindley; Ria Thomas; Jason A Davis; Michela Deleidi; Thomas Gasser; Erdal Karaöz; Jan Pruszak Journal: PLoS One Date: 2013-06-24 Impact factor: 3.240
Authors: Kenneth T Kotz; Wenzong Xiao; Carol Miller-Graziano; Wei-Jun Qian; Aman Russom; Elizabeth A Warner; Lyle L Moldawer; Asit De; Paul E Bankey; Brianne O Petritis; David G Camp; Alan E Rosenbach; Jeremy Goverman; Shawn P Fagan; Bernard H Brownstein; Daniel Irimia; Weihong Xu; Julie Wilhelmy; Michael N Mindrinos; Richard D Smith; Ronald W Davis; Ronald G Tompkins; Mehmet Toner Journal: Nat Med Date: 2010-08-29 Impact factor: 53.440