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Literature DB >> 1579803

Inhibiting effect of charged polymers on interaction of human lupus autoantibodies with certain intracellular proteins.

A E Kabakov¹, L N Brovkina, A M Poverenny, L Cebecauer.

Abstract

The influence of charged polymers on the reaction of immunoglobulins from human lupus sera with cellular proteins was investigated in this study. Through immunoblotting it was shown that polyanions (dextran sulfate, heparin, polyinosinic acid) and polylysine inhibited autoantibody binding to several polypeptides of different molecular mass. Using immunofluorescent staining with affinity isolated monospecific autoantibodies it was demonstrated that the immunoreactivity of two nuclear antigens (p30 and p85) and one cytoplasmic antigen (p40) was sensitive to the presence of charged polymers. The inhibiting effect correlated with the concentration of the polymers. The data obtained suggested the competitive mechanism of inhibition of autoantibody-protein reaction by the charged polymers.

Entities: Chemical Gene Species

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Year: 1992 PMID： 1579803 DOI： 10.1007/bf00301498

Source DB: PubMed Journal: Rheumatol Int ISSN： 0172-8172 Impact factor: 2.631

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Inhibiting effect of charged polymers on interaction of human lupus autoantibodies with certain intracellular proteins.

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