Literature DB >> 1579445

Aminoacyl-tRNA synthetase-induced cleavage of tRNA.

S Beresten1, M Jahn, D Söll.   

Abstract

Aminoacyl-tRNA synthetases interact with their cognate tRNAs in a highly specific fashion. We have examined the phenomenon that upon complex formation E. coli glutaminyl-tRNA synthetase destabilizes tRNA(Gln) causing chain scissions in the presence of Mg2+ ions. The phosphodiester bond cleavage produces 3'-phosphate and 5'-hydroxyl ends. This kind of experiment is useful for detecting conformational changes in tRNA. Our results show that the cleavage is synthetase-specific, that mutant and wild-type tRNA(Gln) species can assume a different conformation, and that modified nucleosides in tRNA enhance the structural stability of the molecule.

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Year:  1992        PMID： 1579445      PMCID： PMC312233          DOI： 10.1093/nar/20.7.1523

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  Codon and amino-acid specificities of a transfer RNA are both converted by a single post-transcriptional modification.

Authors:  T Muramatsu; K Nishikawa; F Nemoto; Y Kuchino; S Nishimura; T Miyazawa; S Yokoyama
Journal:  Nature       Date:  1988-11-10       Impact factor: 49.962

2.  Conformational changes and dynamics of tRNAs: evidence from hydrolysis patterns.

Authors:  A C Dock-Bregeon; D Moras
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1987

3.  Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro.

Authors:  J R Sampson; O C Uhlenbeck
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

4.  Comparison of the tertiary structure of yeast tRNA(Asp) and tRNA(Phe) in solution. Chemical modification study of the bases.

Authors:  P Romby; D Moras; P Dumas; J P Ebel; R Giegé
Journal:  J Mol Biol       Date:  1987-05-05       Impact factor: 5.469

Review 5.  Transfer RNA modification.

Authors:  G R Björk; J U Ericson; C E Gustafsson; T G Hagervall; Y H Jönsson; P M Wikström
Journal:  Annu Rev Biochem       Date:  1987       Impact factor: 23.643

6.  Glutaminyl-tRNA synthetase of Escherichia coli.

Authors:  P Hoben; D Söll
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

7.  Structure of E. coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP at 2.8 A resolution.

Authors:  M A Rould; J J Perona; D Söll; T A Steitz
Journal:  Science       Date:  1989-12-01       Impact factor: 47.728

8.  Solution conformation of several free tRNALeu species from bean, yeast and Escherichia coli and interaction of these tRNAs with bean cytoplasmic Leucyl-tRNA synthetase. A phosphate alkylation study with ethylnitrosourea.

Authors:  A Dietrich; P Romby; L Maréchal-Drouard; P Guillemaut; R Giegé
Journal:  Nucleic Acids Res       Date:  1990-05-11       Impact factor: 16.971

9.  Yeast tRNAAsp tertiary structure in solution and areas of interaction of the tRNA with aspartyl-tRNA synthetase. A comparative study of the yeast phenylalanine system by phosphate alkylation experiments with ethylnitrosourea.

Authors:  P Romby; D Moras; M Bergdoll; P Dumas; V V Vlassov; E Westhof; J P Ebel; R Giegé
Journal:  J Mol Biol       Date:  1985-08-05       Impact factor: 5.469

10.  Structural basis for misaminoacylation by mutant E. coli glutaminyl-tRNA synthetase enzymes.

Authors:  J J Perona; R N Swanson; M A Rould; T A Steitz; D Söll
Journal:  Science       Date:  1989-12-01       Impact factor: 47.728
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  4 in total

1.  Mosaic tile model for tRNA-enzyme recognition.

Authors:  S V Steinberg; L L Kisselev
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

2.  Probing structural differences between native and in vitro transcribed Escherichia coli valine transfer RNA: evidence for stable base modification-dependent conformers.

Authors:  W B Derrick; J Horowitz
Journal:  Nucleic Acids Res       Date:  1993-10-25       Impact factor: 16.971

3.  The long extra arms of human tRNA((Ser)Sec) and tRNA(Ser) function as major identify elements for serylation in an orientation-dependent, but not sequence-specific manner.

Authors:  X Q Wu; H J Gross
Journal:  Nucleic Acids Res       Date:  1993-12-11       Impact factor: 16.971

4.  Recognition of bases in Escherichia coli tRNA(Gln) by glutaminyl-tRNA synthetase: a complete identity set.

Authors:  Y Hayase; M Jahn; M J Rogers; L A Sylvers; M Koizumi; H Inoue; E Ohtsuka; D Söll
Journal:  EMBO J       Date:  1992-11       Impact factor: 11.598
  4 in total

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