| Literature DB >> 15793356 |
Aifen Lin1, Eun-Hee Shin, Tae-Yun Kim, Jae-Hwan Park, Sang-Mee Guk, Jong-Yil Chai.
Abstract
Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5o and 3o ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.Entities:
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Year: 2005 PMID： 15793356 PMCID： PMC2712006 DOI： 10.3347/kjp.2005.43.1.27
Source DB: PubMed Journal: Korean J Parasitol ISSN： 0023-4001 Impact factor: 1.341