Susceptibility to antibiotics and beta-lactamase induction in murein hydrolase mutants of Escherichia coli.

The antibiotic susceptibilities and capabilities to induce beta-lactamases were studied in multiple Escherichia coli murein (peptidoglycan) hydrolase mutants. E. coli mutants lacking either three amidases, three amidases and one lytic transglycosylase, or six lytic transglycosylases showed higher levels of susceptibility to bacitracin, erythromycin, gallidermin, and vancomycin than the wild type. Mutant cells without three amidases lost viability in the presence of vancomycin and gallidermin, whereas the wild type was resistant to both antibiotics. Beta-lactamase induction was studied after introduction of a plasmid carrying the ampC and ampR genes. Upon addition of cefoxitin to the growth medium, the wild type as well as a mutant lacking all known amidases and DD-endopeptidases induced beta-lactamase, whereas a mutant lacking all known lytic transglycosylases was unable to induce beta-lactamase, showing that lytic transglycosylase activity is essential for beta-lactamase induction. Consequently, cells lacking lytic transglycosylase activity lysed in the presence of penicillin, despite the presence of the inducible beta-lactamase system. We discuss the potential of murein hydrolase inhibitors for antibiotic therapy.