Comparative study of blood cell staining with wright-giemsa stain, field stain, and a new modified stain.

Routine staining of blood films usually entails staining with Wright-Giemsa stain; however, some modifications of the standard Wright-Giemsa stain have been performed. The field stain or dip method is an example of these modifications. The authors created a new modification of Wright-Giemsa stain and performed a study to compare our modified stain with Wright-Giemsa stain and field stain. Three experts in microscopy were used in performing this comparative study. The average grading score from each stain method from all 3 experts was set as a basic parameter for comparison. We found that the field stain, which was created for field work, had a lower grading score in staining than the other 2 staining methods in many studied aspects (analysis of variance test, P < .05). In addition, we can demonstrate that the 3 experts presented similar decisions on the blood smear evaluation. Conclusively, we found that our modified method can give staining results similar to the standard Wright-Giemsa stain. In addition, our modification can decrease the staining time.