Literature DB >> 15789263

Generation of recombinant norovirus-like particles (VLP) in the human endothelial kidney cell line 293T.

S Taube1, A Kurth, E Schreier.   

Abstract

Noroviruses (NVs) are the major cause of non-bacterial outbreaks of gastroenteritis. Here we report a new alternative system to generate recombinant NV virus-like particles (rNV-VLP) in a human endothelial kidney cell line (HEK). Transfecting HEK-293T cells with an expression vector coding for the ORF-2 gene lead to the expression of the viral structural protein VP1 which spontaneously assembled into virus-like particles (VLP), as shown by electron microscopy. The transfected cells did not show a cytopathic effect and released rNV-VLP into the culture medium. The HEK-293T cell derived particles were morphologically indistinguishable to the rNV-VLP produced from baculovirus and the Venezuelan equine encephalitis virus (VEE)-replicon. The produced particles were stable for at least 2.5 months at 4 degrees C.

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Year:  2005        PMID： 15789263     DOI： 10.1007/s00705-005-0517-x

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  14 in total

1.  High-resolution x-ray structure and functional analysis of the murine norovirus 1 capsid protein protruding domain.

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Journal:  J Virol       Date:  2010-03-24       Impact factor: 5.103

2.  Model systems for the study of human norovirus Biology.

Authors:  S Vashist; D Bailey; A Putics; I Goodfellow
Journal:  Future Virol       Date:  2009-07       Impact factor: 1.831

Review 3.  Norovirus P particle: a subviral nanoparticle for vaccine development against norovirus, rotavirus and influenza virus.

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Journal:  Nanomedicine (Lond)       Date:  2012-06       Impact factor: 5.307

Review 4.  Vaccine against norovirus.

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Journal:  Hum Vaccin Immunother       Date:  2014-05-05       Impact factor: 3.452

5.  Murine noroviruses bind glycolipid and glycoprotein attachment receptors in a strain-dependent manner.

Authors:  Stefan Taube; Jeffrey W Perry; Eoghan McGreevy; Kristen Yetming; Cheryl Perkins; Kenneth Henderson; Christiane E Wobus
Journal:  J Virol       Date:  2012-03-21       Impact factor: 5.103

6.  Vesicular stomatitis virus as a vector to deliver virus-like particles of human norovirus: a new vaccine candidate against an important noncultivable virus.

Authors:  Yuanmei Ma; Jianrong Li
Journal:  J Virol       Date:  2011-01-12       Impact factor: 5.103

7.  Expression and self-assembly of virus-like particles from two genotypes of marine vesiviruses and development of an ELISA for the detection of antibodies.

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8.  Human Noroviruses Attach to Intestinal Tissues of a Broad Range of Animal Species.

Authors:  Nele Villabruna; Claudia M E Schapendonk; Georgina I Aron; Marion P G Koopmans; Miranda de Graaf
Journal:  J Virol       Date:  2021-01-13       Impact factor: 5.103

9.  Glycosphingolipids as receptors for non-enveloped viruses.

Authors:  Stefan Taube; Mengxi Jiang; Christiane E Wobus
Journal:  Viruses       Date:  2010-04-15       Impact factor: 5.818

Review 10.  The Antigenic Topology of Norovirus as Defined by B and T Cell Epitope Mapping: Implications for Universal Vaccines and Therapeutics.

Authors:  Jessica M van Loben Sels; Kim Y Green
Journal:  Viruses       Date:  2019-05-10       Impact factor: 5.048
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