| Literature DB >> 15782938 |
D Q Liu1, H Liu, X L Gao, D J Leak, N Y Zhou.
Abstract
3-Hydroxybenzoate 6-hydroxylase from Klebsiella pneumoniae M5a1 is an enzyme that utilizes 3-hydroxybenzoate (3-HBA) as substrate yielding gentisate. Site-directed mutagenesis was carried out to define which residues may be involved in catalytic reaction. Substitution of arginine to glutamate at position 169 of the enzyme resulted in the complete loss of catalytic activity. This indicated Arg169 may play an important role in 3-HBA 6-hydroxylase catalysis.Entities:
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Year: 2005 PMID： 15782938 DOI： 10.1016/j.micres.2004.09.003
Source DB: PubMed Journal: Microbiol Res ISSN： 0944-5013 Impact factor: 5.415