Ultrastructure of the horseshoe bat's organ of Corti. I. Scanning electron microscopy.

The organ of Corti of the echolocating horseshoe bat (Rhinolophus rouxi) was investigated with scanning electron microscopy in order to provide a comparison with non-echolocating mammals. Throughout the cochlea of horseshoe bats, each outer hair cell (OHC) possesses three rows of stereocilia and there are no morphological distinctions among the different rows of OHCs. However, there are morphological differences between different regions along the cochlea. In the lower and upper basal turn, the receptor surfaces of OHCs are characterized by extremely wide W-shaped stereocilia bundles and wingshaped cuticular plates. The cuticular plates of OHCs of the middle and outermost rows are arranged parallel to each other. Stereocilia length is only 0.8 microns and there is an exaggerated angle of inclination of the shortest row of stereocilia towards the next taller one. Stereocilia arrangements in the apex of the horseshoe bat's cochlea closely resembles those observed in the midbasal region of the rat cochlea. Inner hair cells (IHC) in the lower basal turn appear specialized. They possess only two rows of stereocilia and only 7-8 stereocilia per row. Their cuticular plates are small and oval and widely separated from one another in the longitudinal direction. IHCs at all other locations possess three and up to four rows of stereocilia and 17-20 stereocilia per row. Their cuticular plates are elongated and closely spaced. The transition from specialized to typical mammalian morphology occurs abruptly (over a distance of about 100-150 microns) at the border between the lower and the upper basal turn. This transition is not accompanied by a change in OHC morphology. In the subsurface of the tectorial membrane, throughout the cochlea, there are distinct imprints of the tallest row of stereocilia of all three rows of OHCs and of the IHCs. Data are discussed in relation to specialized aspects of the cochlear frequency map in horseshoe bats and as possible micromechanical adaptations to ultra-high frequency hearing.