| Literature DB >> 1577914 |
K S Epler1, L C Sander, R G Ziegler, S A Wise, N E Craft.
Abstract
Sixty commercially available and five experimental liquid chromatography columns were evaluated for the separation and recovery of seven carotenoid compounds. Methanol- and acetonitrile-based solvents (either straight or modified with ethyl acetate or tetrahydrofuran) were compared to determine which solvent systems and which columns provided better selectivity and recovery. Methanol-based solvents typically provided higher recoveries than did acetonitrile-based solvents. Polymeric C18 phases generally provided better selectivity for the difficult separation of lutein and zeaxanthin than did monomeric C18 phases.Entities:
Mesh:
Substances:
Year: 1992 PMID: 1577914 DOI: 10.1016/0021-9673(92)85149-n
Source DB: PubMed Journal: J Chromatogr