Literature DB >> 1577914

Evaluation of reversed-phase liquid chromatographic columns for recovery and selectivity of selected carotenoids.

K S Epler1, L C Sander, R G Ziegler, S A Wise, N E Craft.   

Abstract

Sixty commercially available and five experimental liquid chromatography columns were evaluated for the separation and recovery of seven carotenoid compounds. Methanol- and acetonitrile-based solvents (either straight or modified with ethyl acetate or tetrahydrofuran) were compared to determine which solvent systems and which columns provided better selectivity and recovery. Methanol-based solvents typically provided higher recoveries than did acetonitrile-based solvents. Polymeric C18 phases generally provided better selectivity for the difficult separation of lutein and zeaxanthin than did monomeric C18 phases.

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Year:  1992        PMID: 1577914     DOI: 10.1016/0021-9673(92)85149-n

Source DB:  PubMed          Journal:  J Chromatogr


  3 in total

1.  Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis.

Authors:  Jens Steinbrenner; Gerhard Sandmann
Journal:  Appl Environ Microbiol       Date:  2006-09-29       Impact factor: 4.792

2.  Phytochemical and biological studies of Butia capitata Becc. leaves cultivated in Egypt.

Authors:  Nagwa Mohamed Ammar; Mohammed Said Hefnawy; Sahar Youssef Al-Okbi; Doha Abdou Mohamed; Nabil Khamis El-Sayed; Amira Ahmed El-Anssary; Tom Mabry
Journal:  Asian Pac J Trop Biomed       Date:  2014-06

3.  Certification of fat-soluble vitamins, carotenoids, and cholesterol in human serum: Standard Reference Material 968b.

Authors:  J B Thomas; M C Kline; S B Schiller; P M Ellerbe; L T Sniegoski; D L Duewer; K E Sharpless
Journal:  Anal Bioanal Chem       Date:  1996-08       Impact factor: 4.142

  3 in total

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