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Literature DB >> 15774887

The relA homolog of Mycobacterium smegmatis affects cell appearance, viability, and gene expression.

John L Dahl¹, Kriti Arora, Helena I Boshoff, Danelle C Whiteford, Sophia A Pacheco, Olaus J Walsh, Dalia Lau-Bonilla, William B Davis, Anthony G Garza.

Abstract

The modification of metabolic pathways to allow for a dormant lifestyle appears to be an important feature for the survival of pathogenic bacteria within their host. One regulatory mechanism for persistent Mycobacterium tuberculosis infections is the stringent response. In this study, we analyze the stringent response of a nonpathogenic, saprophytic mycobacterial species, Mycobacterium smegmatis. The use of M. smegmatis as a tool for studying the mycobacterial stringent response was demonstrated by measuring the expression of two M. tuberculosis genes, hspX and eis, in M. smegmatis in the presence and absence of rel(Msm). The stringent response plays a role in M. smegmatis cellular and colony formation that is suggestive of changes in the bacterial cell wall structure.

Entities: Disease Species

Mesh：

Substances：

Year: 2005 PMID： 15774887 PMCID： PMC1065248 DOI： 10.1128/JB.187.7.2439-2447.2005

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

36 in total

1. Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement.

Authors: T Parish; N G Stoker
Journal: Microbiology Date: 2000-08 Impact factor: 2.777

2. DksA: a critical component of the transcription initiation machinery that potentiates the regulation of rRNA promoters by ppGpp and the initiating NTP.

Authors: Brian J Paul; Melanie M Barker; Wilma Ross; David A Schneider; Cathy Webb; John W Foster; Richard L Gourse
Journal: Cell Date: 2004-08-06 Impact factor: 41.582

3. Regulation through the secondary channel--structural framework for ppGpp-DksA synergism during transcription.

Authors: Anna Perederina; Vladimir Svetlov; Marina N Vassylyeva; Tahir H Tahirov; Shigeyuki Yokoyama; Irina Artsimovitch; Dmitry G Vassylyev
Journal: Cell Date: 2004-08-06 Impact factor: 41.582

4. Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.

Authors: H Xiao; M Kalman; K Ikehara; S Zemel; G Glaser; M Cashel
Journal: J Biol Chem Date: 1991-03-25 Impact factor: 5.157

Review 5. Molecular basis of bacterial outer membrane permeability.

Authors: H Nikaido; M Vaara
Journal: Microbiol Rev Date: 1985-03

6. Subcellular localization of the Iitracellular survival-enhancing Eis protein of Mycobacterium tuberculosis.

Authors: J L Dahl; J Wei; J W Moulder; S Laal; R L Friedman
Journal: Infect Immun Date: 2001-07 Impact factor: 3.441

7. A rapid and gentle method for the isolation of genomic DNA from mycobacteria.

Authors: M Bose; A Chander; R H Das
Journal: Nucleic Acids Res Date: 1993-05-25 Impact factor: 16.971

8. The 16-kDa alpha-crystallin (Acr) protein of Mycobacterium tuberculosis is required for growth in macrophages.

Authors: Y Yuan; D D Crane; R M Simpson; Y Q Zhu; M J Hickey; D R Sherman; C E Barry
Journal: Proc Natl Acad Sci U S A Date: 1998-08-04 Impact factor: 11.205

9. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice.

Authors: John L Dahl; Carl N Kraus; Helena I M Boshoff; Bernard Doan; Korrie Foley; David Avarbock; Gilla Kaplan; Valerie Mizrahi; Harvey Rubin; Clifton E Barry
Journal: Proc Natl Acad Sci U S A Date: 2003-08-01 Impact factor: 11.205

10. Adaptation of Mycobacterium smegmatis to stationary phase.

Authors: M J Smeulders; J Keer; R A Speight; H D Williams
Journal: J Bacteriol Date: 1999-01 Impact factor: 3.490

31 in total

1. Regulation of Growth, Cell Shape, Cell Division, and Gene Expression by Second Messengers (p)ppGpp and Cyclic Di-GMP in Mycobacterium smegmatis.

Authors: Kuldeepkumar Ramnaresh Gupta; Priyanka Baloni; Shantinath S Indi; Dipankar Chatterji
Journal: J Bacteriol Date: 2016-04-14 Impact factor: 3.490

The relA homolog of Mycobacterium smegmatis affects cell appearance, viability, and gene expression.

1. Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement.

2. DksA: a critical component of the transcription initiation machinery that potentiates the regulation of rRNA promoters by ppGpp and the initiating NTP.

3. Regulation through the secondary channel--structural framework for ppGpp-DksA synergism during transcription.

4. Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.

Review 5. Molecular basis of bacterial outer membrane permeability.

6. Subcellular localization of the Iitracellular survival-enhancing Eis protein of Mycobacterium tuberculosis.

7. A rapid and gentle method for the isolation of genomic DNA from mycobacteria.

8. The 16-kDa alpha-crystallin (Acr) protein of Mycobacterium tuberculosis is required for growth in macrophages.

9. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice.

10. Adaptation of Mycobacterium smegmatis to stationary phase.

1. Regulation of Growth, Cell Shape, Cell Division, and Gene Expression by Second Messengers (p)ppGpp and Cyclic Di-GMP in Mycobacterium smegmatis.

2. Essential roles for Mycobacterium tuberculosis Rel beyond the production of (p)ppGpp.

3. Molecular dissection of the mycobacterial stringent response protein Rel.

Review 4. Bacterial lifestyle shapes stringent response activation.

5. Analyzing the regulatory role of the HigA antitoxin within Mycobacterium tuberculosis.

6. Unusual regioversatility of acetyltransferase Eis, a cause of drug resistance in XDR-TB.

7. Recombinant reporter assay using transcriptional machinery of Mycobacterium tuberculosis.

8. MS_RHII-RSD, a dual-function RNase HII-(p)ppGpp synthetase from Mycobacterium smegmatis.

9. Inactivation of lsr2 results in a hypermotile phenotype in Mycobacterium smegmatis.

10. Evolution of Mycolic Acid Biosynthesis Genes and Their Regulation during Starvation in Mycobacterium tuberculosis.