Asanoa iriomotensis sp. nov., isolated from mangrove soil.

During a study of the distribution of actinomycetes in a mangrove zone, a strain forming spore chains borne on the tip of short sporophores arising directly from the agar surface was isolated from soil. The isolate contained glutamic acid, glycine, alanine and meso-diaminopimelic acid as cell-wall amino acids, menaquinone MK-10(H(6), H(8)), fatty acid type 2d and xylose in the whole-cell hydrolysate. The 16S rRNA gene sequence of the isolate formed a monophyletic cluster with the members of the genus Asanoa in the family Micromonosporaceae. On the basis of morphological and chemotaxonomic characteristics, phylogenetic analysis and DNA-DNA hybridization, a novel species of the genus Asanoa is proposed for strain TT 97-02(T) (=NBRC 100142(T)=DSM 44745(T)), Asanoa iriomotensis sp. nov.