OBJECTIVE: The formation and growth of nasal polyps requires remodeling of the extracellular matrix. Matrix metalloproteinases (MMPs) are a family of endopeptidases that can collectively degrade almost all extracellular matrix components. The aim of this study was to investigate the role of MMPs in nasal polyps. MATERIAL AND METHODS: The expression of MMP-2 and -9 was investigated in the nasal polyps of 20 patients undergoing endonasal sinus surgery and compared with that in control nasal mucosal samples obtained from 20 non-allergic hypertrophic rhinitis patients. Tissue samples were either homogenized for reverse transcriptase polymerase chain reaction analysis or frozen for immunohistochemistry. RESULTS: MMP-2 mRNA expression was observed only in the nasal polyp samples and not in the control tissues. MMP-9 mRNA was not expressed in either type of tissue. Similarly, MMP-2 expression was observed in the nasal polyps of chronic sinusitis patients but not in the inferior turbinate mucosa of the control subjects. MMP-2 positivity was present in basal cells in the surface epithelium and in infiltrating cells in subepithelial tissues. MMP-9-positive cells were not observed in either nasal polyps or inferior turbinates. These results suggest that MMP-2 expression is characteristic of nasal polyps. CONCLUSION: MMP-2 may play a role in the remodeling of nasal polyps.
OBJECTIVE: The formation and growth of nasal polyps requires remodeling of the extracellular matrix. Matrix metalloproteinases (MMPs) are a family of endopeptidases that can collectively degrade almost all extracellular matrix components. The aim of this study was to investigate the role of MMPs in nasal polyps. MATERIAL AND METHODS: The expression of MMP-2 and -9 was investigated in the nasal polyps of 20 patients undergoing endonasal sinus surgery and compared with that in control nasal mucosal samples obtained from 20 non-allergic hypertrophic rhinitispatients. Tissue samples were either homogenized for reverse transcriptase polymerase chain reaction analysis or frozen for immunohistochemistry. RESULTS:MMP-2 mRNA expression was observed only in the nasal polyp samples and not in the control tissues. MMP-9 mRNA was not expressed in either type of tissue. Similarly, MMP-2 expression was observed in the nasal polyps of chronic sinusitispatients but not in the inferior turbinate mucosa of the control subjects. MMP-2 positivity was present in basal cells in the surface epithelium and in infiltrating cells in subepithelial tissues. MMP-9-positive cells were not observed in either nasal polyps or inferior turbinates. These results suggest that MMP-2 expression is characteristic of nasal polyps. CONCLUSION:MMP-2 may play a role in the remodeling of nasal polyps.
Authors: Julie A Poposki; Ashraf Uzzaman; Deepti R Nagarkar; Regina T Chustz; Anju T Peters; Lydia A Suh; Roderick Carter; James Norton; Kathleen E Harris; Leslie C Grammer; Bruce K Tan; Rakesh K Chandra; David B Conley; Robert C Kern; Robert P Schleimer; Atsushi Kato Journal: J Allergy Clin Immunol Date: 2011-04-17 Impact factor: 10.793
Authors: Rafael Rossell Malinsky; Fabiana C P Valera; Fransérgio Emílio Cavallari; Daniel Salgado Küpper; Cristiane Milaneze; João S Silva; Edwin Tamashiro; Wilma T Anselmo-Lima Journal: Eur Arch Otorhinolaryngol Date: 2012-10-12 Impact factor: 2.503
Authors: Kara Y Detwiller; Timothy L Smith; Jess C Mace; Dennis R Trune; Nathan B Sautter Journal: Int Forum Allergy Rhinol Date: 2013-02-08 Impact factor: 3.858
Authors: C N Tiboc Schnell; Gabriela Adriana Filip; N Decea; R Moldovan; R Opris; S C Man; B Moldovan; L David; F Tabaran; D Olteanu; A M Gheldiu; I Baldea Journal: Inflammopharmacology Date: 2021-04-21 Impact factor: 4.473