| Literature DB >> 15765589 |
Fabíola Karla Corrêa Ribeiro1, Valdério do Valle Dettoni, Renata Lyrio Peres, Solange Alves Vinhas, Tatiana Resende Có, Reynaldo Dietze, Moisés Palaci.
Abstract
A ligase chain reaction DNA amplification method for direct detection of Mycobacterium tuberculosis (Abbott LCx MTB) in respiratory specimens was evaluated. Results from LCx MTB Assay were compared with those from acid fast bacilli smear, culture, and final clinical diagnosis for each patient. A total of 297 respiratory specimens (sputum and bronchial lavage) from 193 patients were tested. The sensitivity, specificity, positive predictive value and negative predictive value of LC vs culture were 92.7%, 93%, 67.8% and 98.7%, respectively. When compared to the clinical final diagnosis, the sensitivity, specificity, PPV and NPV for LCx were 88.9%, 96.8%, 86.5% and 97.4%, respectively. The sensitivity of LCx MTB assay was 75% for smear-negative, culture positive samples. The results indicate that LCx MTB assay is a rapid, simple and valuable technique as a complementary tool for the diagnosis of tuberculosis.Entities:
Mesh：
Substances：
Year: 2004 PMID： 15765589 DOI： 10.1590/s0037-86822004000600001
Source DB: PubMed Journal: Rev Soc Bras Med Trop ISSN： 0037-8682 Impact factor: 1.581